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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2004-5-3
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pubmed:abstractText |
Myristoylation of ARF family GTPases is required for their association with Golgi and endosomal membranes, where they regulate protein sorting and the lipid composition of these organelles. The Golgi-localized ARF-like GTPase Arl3p/ARP lacks a myristoylation signal, indicating that its targeting mechanism is distinct from myristoylated ARFs. We demonstrate that acetylation of the N-terminal methionine of Arl3p requires the NatC N(alpha)-acetyltransferase and that this modification is required for its Golgi localization. Chemical crosslinking and fluorescence microscopy experiments demonstrate that localization of Arl3p also requires Sys1p, a Golgi-localized integral membrane protein, which may serve as a receptor for acetylated Arl3p.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-Ribosylation Factors,
http://linkedlifedata.com/resource/pubmed/chemical/ARL3 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Acetyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Arylamine N-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/MAK10 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/MAK3 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sys1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1465-7392
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
414-9
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pubmed:dateRevised |
2009-9-7
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pubmed:meshHeading |
pubmed-meshheading:15077114-ADP-Ribosylation Factors,
pubmed-meshheading:15077114-Acetylation,
pubmed-meshheading:15077114-Acetyltransferases,
pubmed-meshheading:15077114-Arylamine N-Acetyltransferase,
pubmed-meshheading:15077114-Golgi Apparatus,
pubmed-meshheading:15077114-Membrane Proteins,
pubmed-meshheading:15077114-Protein Transport,
pubmed-meshheading:15077114-Recombinant Fusion Proteins,
pubmed-meshheading:15077114-Saccharomyces cerevisiae,
pubmed-meshheading:15077114-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:15077114-Vesicular Transport Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p.
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pubmed:affiliation |
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Blvd. BRB 2/3 room 1010, Philadelphia, PA 19104-6058, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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