Linked Life Data

15072830

Source:http://linkedlifedata.com/resource/pubmed/id/15072830

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-4-9
pubmed:abstractText
We investigated a role for Hedgehog signalling in colon cancer by studying transcription of members of the pathway in human colorectal carcinoma cell lines. We determined the methylation status and screened the gene encoding the Hedgehog receptor-associated protein Smoothened (SMO) for putative mutations. In three cell lines lacking SMO expression the SMO promoter was fully methylated and the transcription factor GLI3 was not expressed. Two additional cell lines both having one methylated SMO allele and expressing mutant SMO did not express GLI3. Our results suggest that expression of wild-type SMO is required for expression of GLI3 by a mechanism that is independent of conventional Hedgehog signalling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0304-3835
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
207
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-14
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15072830-Base Sequence, pubmed-meshheading:15072830-Colorectal Neoplasms, pubmed-meshheading:15072830-DNA Methylation, pubmed-meshheading:15072830-DNA-Binding Proteins, pubmed-meshheading:15072830-Heterozygote, pubmed-meshheading:15072830-Humans, pubmed-meshheading:15072830-Kruppel-Like Transcription Factors, pubmed-meshheading:15072830-Molecular Sequence Data, pubmed-meshheading:15072830-Mutation, pubmed-meshheading:15072830-Nerve Tissue Proteins, pubmed-meshheading:15072830-Polymorphism, Single-Stranded Conformational, pubmed-meshheading:15072830-Promoter Regions, Genetic, pubmed-meshheading:15072830-Receptors, G-Protein-Coupled, pubmed-meshheading:15072830-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15072830-Transcription Factors, pubmed-meshheading:15072830-Tumor Cells, Cultured
pubmed:year
2004
pubmed:articleTitle
Functional Smoothened is required for expression of GLI3 in colorectal carcinoma cells.
pubmed:affiliation
Sir Alastair Currie Cancer Research UK Laboratories, Division of Pathology, Molecular Medicine Centre, University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't