Linked Life Data

15072590

Source:http://linkedlifedata.com/resource/pubmed/id/15072590

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-4-9
pubmed:abstractText
Tumor-stromal interactions, which are regulated by stromal-derived HGF and tumor-derived HGF inducers, are essential for tumor cell acquisition of such malignant properties as invasion and metastasis. NK4, a proteolytic cleavage product of HGF, has antitumor activities as both an HGF antagonist and an angiogenesis inhibitor. In this study, we examined the in vitro and in vivo behaviors of mouse colon adenocarcinoma C T26 cells modified by gene transfer to secrete NK4, and investigated the influence of NK4 on expression of HGF and HGF inducers associated with tumor-stromal interactions. In vitro cell proliferation rates of NK4 transfectant (C T26-NK4) and mock transfectant (C T26-NEO) were essentially the same, and scattering and invasion were stimulated by HGF in C T26-NEO, but not in C T26-NK4. In syngeneic BALB/c female mice, subcutaneous tumor growth of C T26-NK4 was potently suppressed, and the survival was prolonged significantly. Immunohistochemistry showed significantly decreased microvessels and increased apoptotic cells in C T26-NK4 tumor compared with control. Interestingly, HGF, strongly expressed in C T26-NEO tumor stroma, was reduced in C T26-NK4. In vitro, conditioned medium of C T26-NK4 inhibited fibroblast-derived HGF production, which was increased by that of C T26-NEO. Moreover, although similar constitutive expression levels of PDGF and TGF-alpha (both HGF inducers) were detected in C T26-NK4 and C T26-NEO in semiquantitative RT-PCR analyses, the expression was up-regulated by HGF in C T26-NEO, but not C T26-NK4. These results suggest that NK4 may exert antitumor activities not only by antagonizing HGF, but also by inhibiting HGF amplification via tumor-stromal interactions. Continuous, abundant NK4 production induced at a tumor site by gene transfer should show multiple antitumor activities with potential therapeutic benefit.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1347-9032
pubmed:author
pubmed:issnType
Print
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
321-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15072590-Adenocarcinoma, pubmed-meshheading:15072590-Animals, pubmed-meshheading:15072590-Cell Division, pubmed-meshheading:15072590-Cell Line, Tumor, pubmed-meshheading:15072590-Colonic Neoplasms, pubmed-meshheading:15072590-Female, pubmed-meshheading:15072590-Fibroblasts, pubmed-meshheading:15072590-Gene Expression Regulation, Neoplastic, pubmed-meshheading:15072590-Hepatocyte Growth Factor, pubmed-meshheading:15072590-Humans, pubmed-meshheading:15072590-Mice, pubmed-meshheading:15072590-Mice, Inbred BALB C, pubmed-meshheading:15072590-Mitogens, pubmed-meshheading:15072590-Neoplasm Transplantation, pubmed-meshheading:15072590-Phenotype, pubmed-meshheading:15072590-Platelet-Derived Growth Factor, pubmed-meshheading:15072590-Recombinant Proteins, pubmed-meshheading:15072590-Stromal Cells, pubmed-meshheading:15072590-Transfection, pubmed-meshheading:15072590-Transforming Growth Factor alpha
pubmed:year
2004
pubmed:articleTitle
Reduced HGF expression in subcutaneous CT26 tumor genetically modified to secrete NK4 and its possible relation with antitumor effects.
pubmed:affiliation
Department of Digestive Surgery, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-0841, Japan. tkubot@koto.kpu-m.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't