Linked Life Data

15071120

Source:http://linkedlifedata.com/resource/pubmed/id/15071120

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2004-4-8
pubmed:abstractText
Although synapsins are abundant synaptic vesicle proteins that are widely used as markers of presynaptic terminals, the mechanisms that target synapsins to presynaptic terminals have not been elucidated. We have addressed this question by imaging the targeting of green fluorescent protein-tagged synapsins in cultured hippocampal neurons. Whereas all synapsin isoforms targeted robustly to presynaptic terminals in wild-type neurons, synapsin Ib scarcely targeted in neurons in which all synapsins were knocked-out. Coexpression of other synapsin isoforms significantly strengthened the targeting of synapsin Ib in knock-out neurons, indicating that heterodimerization is required for synapsin Ib to target. Truncation mutagenesis revealed that synapsin Ia targets via distributed binding sites that include domains B, C, and E. Although domain A was not necessary for targeting, its presence enhanced targeting. Domain D inhibited targeting, but this inhibition was overcome by domain E. Thus, multiple intermolecular and intramolecular interactions are required for synapsins to target to presynaptic terminals.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3711-20
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15071120-Animals, pubmed-meshheading:15071120-Cells, Cultured, pubmed-meshheading:15071120-Dimerization, pubmed-meshheading:15071120-Gene Targeting, pubmed-meshheading:15071120-Green Fluorescent Proteins, pubmed-meshheading:15071120-Luminescent Proteins, pubmed-meshheading:15071120-Mice, pubmed-meshheading:15071120-Mice, Knockout, pubmed-meshheading:15071120-Mutagenesis, Site-Directed, pubmed-meshheading:15071120-Neurons, pubmed-meshheading:15071120-Phosphorylation, pubmed-meshheading:15071120-Presynaptic Terminals, pubmed-meshheading:15071120-Protein Isoforms, pubmed-meshheading:15071120-Protein Structure, Tertiary, pubmed-meshheading:15071120-Protein Transport, pubmed-meshheading:15071120-Recombinant Fusion Proteins, pubmed-meshheading:15071120-Synapsins, pubmed-meshheading:15071120-Synaptic Vesicles, pubmed-meshheading:15071120-Transfection
pubmed:year
2004
pubmed:articleTitle
Molecular determinants of synapsin targeting to presynaptic terminals.
pubmed:affiliation
Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't