Linked Life Data

15066130

Source:http://linkedlifedata.com/resource/pubmed/id/15066130

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-4-6
pubmed:abstractText
Macrophage inflammatory protein (MIP)-1beta and RANTES (regulated on activation, normal T-cells expressed and secreted) are members of the CC-family of chemokines. Although these two peptides are structurally and functionally related to one another, each exhibits distinct features, which allows it to independently regulate specific aspects of the host inflammatory response. They evoked intense and functionally different febrile responses when applied directly on pyrogen-sensitive cells located in the in the preoptic area of the anterior hypothalamus (POA). The present experiments were carried out to test the central role of CCR5, a functional receptor for MIP-1beta and RANTES, in the febrile responses induced by these chemokines when injected directly into the POA. The microinjection of an equimolecular dose (50 pg) of either MIP-1beta or RANTES into the POA induced a rapid onset; monophasic fever in rats that persisted for a long period. The microinjection of 2.0 microg specific neutralizing antibodies against CCR5 (anti-CCR5) into the POA fails to affect the effects on body temperature induced by MIP-1beta. However, pretreatment with the same dose of anti-CCR5 suppressed the febrile response induced by RANTES given at the same site. The microinjection of control IgG or anti-CCR5 does not affect basal temperature, when administered alone at the same hypothalamic site. The present experiments show that hypothalamic CCR5 are functionally involved in the febrile response induced by RANTES, but not by MIP-1beta. They also suggest the existence of functionally different components in the presumptive primary locus of the thermoregulatory controller, in which both chemotactic cytokines, together other mediators, could play a relevant role in the complex process of fever pathogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0767-3981
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
163-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15066130-Animals, pubmed-meshheading:15066130-Antibodies, pubmed-meshheading:15066130-Body Temperature, pubmed-meshheading:15066130-Cerebrospinal Fluid, pubmed-meshheading:15066130-Chemokine CCL4, pubmed-meshheading:15066130-Chemokine CCL5, pubmed-meshheading:15066130-Chemokines, CC, pubmed-meshheading:15066130-Fever, pubmed-meshheading:15066130-Heating, pubmed-meshheading:15066130-Immunoglobulin G, pubmed-meshheading:15066130-Macrophage Inflammatory Proteins, pubmed-meshheading:15066130-Male, pubmed-meshheading:15066130-Microinjections, pubmed-meshheading:15066130-Preoptic Area, pubmed-meshheading:15066130-Pyrogens, pubmed-meshheading:15066130-Rats, pubmed-meshheading:15066130-Rats, Wistar, pubmed-meshheading:15066130-Receptors, CCR5, pubmed-meshheading:15066130-Recombinant Proteins, pubmed-meshheading:15066130-Stereotaxic Techniques, pubmed-meshheading:15066130-Time Factors
pubmed:year
2004
pubmed:articleTitle
Differential sensitivities of pyrogenic chemokine fevers to CC chemokine receptor 5 antibodies.
pubmed:affiliation
Laboratorio de Farmacología Clínica y Experimental. Unidad de Investigación Fundación Valme, Hospital Universitario Virgen de Valme, E-41014, Seville, Spain.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't