Source:http://linkedlifedata.com/resource/pubmed/id/15065669
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2004-4-6
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| pubmed:abstractText |
A current trend in single-particle electron microscopy is to compute three-dimensional reconstructions with ever-increasing numbers of particles in the data sets. Since manual--or even semi-automated--selection of particles represents a major bottleneck when the data set exceeds several thousand particles, there is growing interest in developing automatic methods for selecting images of individual particles. Except in special cases, however, it has proven difficult to achieve the degree of efficiency and reliability that would make fully automated particle selection a useful tool. The simplest methods such as cross correlation (i.e., matched filtering) do not perform well enough to be used for fully automated particle selection. Geometric properties (area, perimeter-to-area ratio, etc.) and the integrated "mass" of candidate particles are additional factors that could improve automated particle selection if suitable methods of contouring particles could be developed. Another suggestion is that data be always collected as pairs of images, the first taken at low defocus (to capture information at the highest possible resolution) and the second at very high defocus (to improve the visibility of the particle). Finally, it is emphasized that well-annotated, open-access data sets need to be established in order to encourage the further development and validation of methods for automated particle selection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1047-8477
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
145
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
15-8
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:15065669-Algorithms,
pubmed-meshheading:15065669-Automatic Data Processing,
pubmed-meshheading:15065669-Image Processing, Computer-Assisted,
pubmed-meshheading:15065669-Imaging, Three-Dimensional,
pubmed-meshheading:15065669-Macromolecular Substances,
pubmed-meshheading:15065669-Microscopy, Electron,
pubmed-meshheading:15065669-Molecular Conformation,
pubmed-meshheading:15065669-Particle Size
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| pubmed:articleTitle |
Historical background: Why is it important to improve automated particle selection methods?
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| pubmed:affiliation |
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3206, USA. rmglaeser@lbl.gov
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| pubmed:publicationType |
Journal Article
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