Linked Life Data

15065150

Source:http://linkedlifedata.com/resource/pubmed/id/15065150

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-4-5
pubmed:abstractText
An advanced six-ring morpholino backbone c-myc antisense (AVI-4126) was shown to inhibit c-myc expression and intimal hyperplasia after local catheter delivery in a porcine balloon injury model. The purpose of this study was to investigate the effects of an AVI-4126-eluting phosphorylcholine-coated (PC) stent on c-myc expression restenosis and vascular healing after stent implantation in porcine coronary arteries. PC stents were loaded with AVI-4126 using soak trap. Nine pigs underwent AVI-4126 PC coronary stent implantation (two stents/animal). Two to six hours postprocedure, three pigs were sacrificed and stented segments were analyzed by Western blot for c-myc expression. In chronic experiments, six pigs (12 stent sites) were sacrificed at 28 days following intervention and vessels were perfusion-fixed. High-performance liquid chromatography analysis of plasma samples showed minimal presence of the antisense. Western blot analysis of the stented vessels demonstrated inhibition of c-myc expression at 2 and 6 hr after procedure. Quantitative histologic morphometry showed that the neointimal area was significantly reduced (by 40%) in the antisense-coated group compared with control (2.3 +/- 0.7 vs. 3.9 +/- 0.8 mm(2), respectively; P = 0.0077). Immunostaining and electron microscopy demonstrated complete endothelialization, without fibrin deposition, thrombosis, or necrosis in all implanted stents. In the porcine coronary model, an advanced c-myc-eluting PC stent blocked c-myc expression and significantly inhibited myointimal hyperplasia and allowed complete reendothelialization and healing response.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1522-1946
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
518-27
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15065150-Animals, pubmed-meshheading:15065150-Blood Vessel Prosthesis Implantation, pubmed-meshheading:15065150-Coated Materials, Biocompatible, pubmed-meshheading:15065150-Coronary Angiography, pubmed-meshheading:15065150-Coronary Restenosis, pubmed-meshheading:15065150-Coronary Vessels, pubmed-meshheading:15065150-Disease Models, Animal, pubmed-meshheading:15065150-Female, pubmed-meshheading:15065150-Follow-Up Studies, pubmed-meshheading:15065150-Male, pubmed-meshheading:15065150-Models, Cardiovascular, pubmed-meshheading:15065150-Morpholines, pubmed-meshheading:15065150-Morpholinos, pubmed-meshheading:15065150-Phosphorylcholine, pubmed-meshheading:15065150-Proto-Oncogene Proteins c-myc, pubmed-meshheading:15065150-Statistics as Topic, pubmed-meshheading:15065150-Stents, pubmed-meshheading:15065150-Swine, pubmed-meshheading:15065150-Treatment Outcome, pubmed-meshheading:15065150-Tunica Intima
pubmed:year
2004
pubmed:articleTitle
Advanced c-myc antisense (AVI-4126)-eluting phosphorylcholine-coated stent implantation is associated with complete vascular healing and reduced neointimal formation in the porcine coronary restenosis model.
pubmed:affiliation
Lenox Hill Heart and Vascular Institute and Cardiovascular Research Foundation, New York, New York 10021, USA. nkipshidze@lenoxhill.net
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies