Linked Life Data

15063506

Source:http://linkedlifedata.com/resource/pubmed/id/15063506

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-4-5
pubmed:abstractText
The tetrahydropyramidine hydroxyectoine acts as an osmolyte in a range of bacterial species, but its use as a desiccation protectant is less well explored. Recently, it was demonstrated that hydroxyectoine provides effective stabilisation of the Gram-negative species Pseudomonas putida, which is only relatively poorly preserved by the better-characterised protectant, trehalose. It is now shown that hydroxyectoine also protects the paradigmatic bacterium, Escherichia coli: osmotically-preconditioned E. coli dried in hydroxyectoine exhibited a high degree of desiccation tolerance, similar to that achieved using trehalose in this species. Hydroxyectoine is apparently accumulated from hypersaline medium in preference to trehalose biosynthesis, but E. coli loaded with hydroxyectoine in this way showed reduced stability in the dry state. This suggests that, although both hydroxyectoine and trehalose perform equally well as extracellular protectants, trehalose is preferred for intracellular protection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
233
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
347-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
High survival and stability rates of Escherichia coli dried in hydroxyectoine.
pubmed:affiliation
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't