Source:http://linkedlifedata.com/resource/pubmed/id/15063342
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2004-4-5
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pubmed:abstractText |
Rosiglitazone is a thiazolidinedione antihyperglycemic drug used in the treatment of type 2 diabetes mellitus. Rosiglitazone is extensively metabolized by cytochrome P450 2C8 and so may have some utility as an in vivo probe for this enzyme. A liquid chromatographic method using sensitive fluorescence detection and simplified sample processing involving protein precipitation with acetonitrile was developed. The isocratic mobile phase consisted of 10 mM sodium acetate-acetonitrile (pH 5; 60:40, v/v) and was delivered at a flow rate of 1 ml/min to an Alltima phenyl column (250 mm x 4.6 mm, 5 microm). Detection was by fluorescence at (EX/EM) 247/367 for rosiglitazone and 235/310 for the internal standard betaxolol. Intra- and inter-day precision ranged from 3.1 to 8.5% and 2.3 to 5.7%, respectively. No endogenous interference was observed with either rosiglitazone or the internal standard. The assay is simple, economical, precise, and is directly applicable to human pharmacokinetic studies involving single dose rosiglitazone administration.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1570-0232
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
803
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
317-20
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15063342-Chromatography, High Pressure Liquid,
pubmed-meshheading:15063342-Humans,
pubmed-meshheading:15063342-Hypoglycemic Agents,
pubmed-meshheading:15063342-Reference Standards,
pubmed-meshheading:15063342-Reproducibility of Results,
pubmed-meshheading:15063342-Sensitivity and Specificity,
pubmed-meshheading:15063342-Spectrometry, Fluorescence,
pubmed-meshheading:15063342-Thiazolidinediones
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pubmed:year |
2004
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pubmed:articleTitle |
Simplified method for determination of rosiglitazone in human plasma.
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pubmed:affiliation |
Department of Pharmaceutical Sciences, Pharmacodynamic Research Center, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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