Source:http://linkedlifedata.com/resource/pubmed/id/15062981
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2004-4-5
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| pubmed:abstractText |
Insulin-like growth factor-1 and -2 (IGFs) are important for CNS development and have implications in pathological situations of the brain. Insulin-like growth factor binding protein-4 (IGFBP-4) regulates the biological effects of IGFs. We examined the expression of IGFBP-4 in primary rat and human astrocytes. IGFBP-4 mRNA was detectable by reverse transcription-polymerase chain reaction (RT-PCR) and protein expression was verified by Western blotting of cell lysates as well as conditioned culture medium. When astrocytes were immunostained for IGFBP-4 we detected an intracellular structure that did not derive from organelles involved in cellular trafficking. Ingestion of fluorescein isothiocyanate-labeled transferrin excluded detection of IGFBP-4 within vesicles of endosomal nature, in which the protein might have been incorporated. Double staining with gamma-tubulin demonstrated co-localization of IGFBP-4 with centrosomes of these cells. Treatment with nocodazole resulted in absence of IGFBP-4 signal on centrosomes, indicating a dependency on intact microtubules. Immunoelectron microscopy revealed IGFBP-4 localization not only at the centrioles but also a direct interaction with microtubules. There was no binding of IGFBP-4 to centrioles in primary rat oligodendrocytes, microglia or meningeal cells. The association of IGFBP-4 with centrioles and microtubules in astrocytes suggests an involvement of this molecule in microtubule functions of these cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Nocodazole,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transferrin,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0306-4522
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
125
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
381-90
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:15062981-Animals,
pubmed-meshheading:15062981-Animals, Newborn,
pubmed-meshheading:15062981-Astrocytes,
pubmed-meshheading:15062981-Blotting, Western,
pubmed-meshheading:15062981-Centrosome,
pubmed-meshheading:15062981-Cerebral Cortex,
pubmed-meshheading:15062981-Fluorescein-5-isothiocyanate,
pubmed-meshheading:15062981-Fluorescent Antibody Technique,
pubmed-meshheading:15062981-Insulin-Like Growth Factor Binding Protein 4,
pubmed-meshheading:15062981-Microscopy, Electron,
pubmed-meshheading:15062981-Microtubules,
pubmed-meshheading:15062981-Nocodazole,
pubmed-meshheading:15062981-RNA, Messenger,
pubmed-meshheading:15062981-Rats,
pubmed-meshheading:15062981-Rats, Wistar,
pubmed-meshheading:15062981-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15062981-Transferrin,
pubmed-meshheading:15062981-Tubulin
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| pubmed:year |
2004
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| pubmed:articleTitle |
Insulin-like growth factor binding protein-4 interacts with centrosomes and microtubules in primary astrocytes.
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| pubmed:affiliation |
Department of Neurology, Academic Hospital Groningen, Hanzeplein 1, 9700 RB Groningen, The Netherlands. d.chesnik@med.rug.nl
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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