Source:http://linkedlifedata.com/resource/pubmed/id/15059007
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2004-4-2
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pubmed:abstractText |
We used real-time multiple particle tracking to quantitatively characterize the type and rates of transport of gene nanocarriers within live cells. The heterogeneous cytoplasmic transport of polyethylenimine (PEI)/DNA gene carriers was quantified by tracking their mean-square displacements over time and classified into active and nonactive transport populations on the basis of their effective diffusivities versus time. Nonactive gene carriers frequently displayed hop-diffusion trajectories, suggesting a porous cytoplasmic network of flexible biopolymers or sequential attachment and detachment events. Microtubule-dependent active transport of gene carriers resulted in an effective diffusivity 30-fold greater than that of nonactive carriers (at a time scale of 3 s). Compared to nonactive carriers in control cells with intact microtubules, microtubule depolymerization enhanced short-range motion of gene carriers but resulted in similar long-range transport. Multiple particle tracking characterizes gene carrier transport in complex biological environments and, therefore, may be a useful tool in quantifying rate-limiting steps in gene delivery within cells and other biological media.
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pubmed:grant | |
pubmed:keyword | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
8756-7938
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
598-602
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15059007-Animals,
pubmed-meshheading:15059007-Biological Transport,
pubmed-meshheading:15059007-COS Cells,
pubmed-meshheading:15059007-Cercopithecus aethiops,
pubmed-meshheading:15059007-DNA,
pubmed-meshheading:15059007-Diffusion,
pubmed-meshheading:15059007-Drug Delivery Systems,
pubmed-meshheading:15059007-Gene Transfer Techniques,
pubmed-meshheading:15059007-Image Interpretation, Computer-Assisted,
pubmed-meshheading:15059007-Intracellular Space,
pubmed-meshheading:15059007-Microscopy, Fluorescence,
pubmed-meshheading:15059007-Motion,
pubmed-meshheading:15059007-Nanotubes,
pubmed-meshheading:15059007-Polyethyleneimine,
pubmed-meshheading:15059007-Transfection
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pubmed:articleTitle |
Real-time intracellular transport of gene nanocarriers studied by multiple particle tracking.
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pubmed:affiliation |
Department of Biomedical Engineering, The Johns Hopkins University, 720 Rutland Ave., Baltimore, Maryland 21205, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Evaluation Studies,
Validation Studies
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