Linked Life Data

15056483

Source:http://linkedlifedata.com/resource/pubmed/id/15056483

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-4-1
pubmed:abstractText
The serotonin transporter (5-HTT) plays a critical role in the termination of serotonin neurotransmission and represents the prime target for selective serotonin reuptake inhibitors (SSRIs). In the present study, the 5-HTT protein in human peripheral blood lymphocyte was characterized pharmacologically and biochemically. The tricyclic antidepressant drug [(3)H]imipramine, an established ligand for the neuronal and platelet 5-HTT, bound saturably and reversibly to a single population of non-interacting binding sites in fresh human peripheral blood lymphocytes. The affinity of [(3)H]imipramine (K(d)) to the transporter, calculated from association and dissociation kinetic experiments, was similar to that obtained from the equilibrium study. The function of the transporter was studied using high affinity [(3)H]5-HT uptake into fresh lymphocytes. [(3)H]Imipramine binding and [(3)H]5-HT uptake were inhibited by tricyclic antidepressants as well as by SSRIs. Western blot analysis as well as immunoprecipitation analysis revealed labeling of a single protein band of approximately 100 kDa. The presence of the 5-HTT in easily accessible nucleated cells such as peripheral blood lymphocytes might permit molecular genetic studies in mood and anxiety disorder patients, and might enhance the understanding of the different efficacies of antidepressants in depressed patients.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0924-977X
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15056483-Antidepressive Agents, Tricyclic, pubmed-meshheading:15056483-Binding Sites, pubmed-meshheading:15056483-Blotting, Western, pubmed-meshheading:15056483-Carrier Proteins, pubmed-meshheading:15056483-Competitive Bidding, pubmed-meshheading:15056483-Dose-Response Relationship, Drug, pubmed-meshheading:15056483-Humans, pubmed-meshheading:15056483-Imipramine, pubmed-meshheading:15056483-Inhibitory Concentration 50, pubmed-meshheading:15056483-Lymphocytes, pubmed-meshheading:15056483-Membrane Glycoproteins, pubmed-meshheading:15056483-Membrane Transport Proteins, pubmed-meshheading:15056483-Nerve Tissue Proteins, pubmed-meshheading:15056483-Precipitin Tests, pubmed-meshheading:15056483-Serotonin, pubmed-meshheading:15056483-Serotonin Plasma Membrane Transport Proteins, pubmed-meshheading:15056483-Tritium
pubmed:year
2004
pubmed:articleTitle
Biochemical and pharmacological characterization of the serotonin transporter in human peripheral blood lymphocytes.
pubmed:affiliation
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't