Linked Life Data

15055447

Source:http://linkedlifedata.com/resource/pubmed/id/15055447

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-4-1
pubmed:abstractText
Behavioral assessments of hindlimb motor recovery and anatomical assessments of extended axons of long spinal tracts were conducted in adult rats following complete spinal cord transection. Rats were randomly divided into 3 groups: 1) sham control group (laminectomy only; n = 12); 2) transection-only group, spinal cord transection at T8 (n = 20); and 3) experimental treatment group, spinal cord transection at T8, with peripheral nerve grafts (PNG) and application of acidic fibroblast growth factor (aFGF) (n = 14). The locomotor behavior and stepping of all rats were analyzed over a 6-month survival time using the Basso, Beattie, Bresnahan (BBB) open field locomotor test and the contact placing test. Immunohistochemistry for serotonin (5-HT), anterograde tracing with biotinylated dextran amine (BDA), and retrograde tracing with fluoro-gold were used to evaluate the presence of axons below the damage site following treatment. When compared with the transection-only group, the nerve graft with the aFGF group showed 1) significant improvement in hindlimb locomotion and stepping, 2) the presence of 5-HT-labeled axons below the lesion site at lumbar cord level (these were interpreted as regenerated axons from the raphe nuclei), 3) the presence of anterograde BDA labeling of corticospinal tract axons at the graft site and below, and 4) fluoro-gold retrograde labeling of neuron populations in motor cortex and in red nucleus, reticulospinal nuclei, raphe nuclei, and vestibular nuclei. We conclude that peripheral nerve grafts and aFGF treatments facilitate the regrowth of the spinal axons and improve hindlimb function in a T-8 spinal cord-transected rat model.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3069
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15055447-Animals, pubmed-meshheading:15055447-Axonal Transport, pubmed-meshheading:15055447-Biotin, pubmed-meshheading:15055447-Dextrans, pubmed-meshheading:15055447-Efferent Pathways, pubmed-meshheading:15055447-Female, pubmed-meshheading:15055447-Fibroblast Growth Factors, pubmed-meshheading:15055447-Fluorescent Dyes, pubmed-meshheading:15055447-Graft Survival, pubmed-meshheading:15055447-Growth Cones, pubmed-meshheading:15055447-Motor Activity, pubmed-meshheading:15055447-Nerve Regeneration, pubmed-meshheading:15055447-Peripheral Nerves, pubmed-meshheading:15055447-Raphe Nuclei, pubmed-meshheading:15055447-Rats, pubmed-meshheading:15055447-Rats, Sprague-Dawley, pubmed-meshheading:15055447-Recovery of Function, pubmed-meshheading:15055447-Serotonin, pubmed-meshheading:15055447-Spinal Cord Injuries, pubmed-meshheading:15055447-Stilbamidines, pubmed-meshheading:15055447-Tissue Transplantation, pubmed-meshheading:15055447-Treatment Outcome
pubmed:year
2004
pubmed:articleTitle
Motor recovery and anatomical evidence of axonal regrowth in spinal cord-repaired adult rats.
pubmed:affiliation
Department of Anatomy, College of Medicine, University of California, Irvine, Irvine, California, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't