Linked Life Data

15048973

Source:http://linkedlifedata.com/resource/pubmed/id/15048973

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-3-29
pubmed:abstractText
The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demonstrated that this interference increases with time between silver staining and gel processing, although the silver image is constant. This suggested an important role of the formaldehyde used in silver staining development in this interference process. Consequently, a formaldehyde-free staining protocol has been devised, using carbohydrazide as the developing agent. This protocol showed much increased peptide coverage and retained the sensitivity of silver staining. These results were however obtained at the expense of an increased background in the stained gels and of a reduced staining homogeneity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1615-9853
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
909-16
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
About the mechanism of interference of silver staining with peptide mass spectrometry.
pubmed:affiliation
Laboratoire de Spectrométrie de Masse Bio-Organique, UMR CNRS 7509, ECPM, Strasbourg, France.
pubmed:publicationType
Journal Article