Source:http://linkedlifedata.com/resource/pubmed/id/15043153
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2004-3-26
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pubmed:abstractText |
We have used the synthesis and 15N NMR study of separate loop A and loop B domains of the hairpin ribozyme to demonstrate that multiple 15N atoms can be incorporated into an RNA strand and be unambiguously distinguished through a combination of direct and indirect tagging by 13C atoms. Absence of 15N chemical shift changes shows that the G8N1 in loop A does not become deprotonated up to pH 8, and that the G21N7 of loop B does not bind to Mg2+.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1525-7770
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
273-80
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15043153-Carbon Isotopes,
pubmed-meshheading:15043153-Hydrogen-Ion Concentration,
pubmed-meshheading:15043153-Magnetic Resonance Spectroscopy,
pubmed-meshheading:15043153-Models, Molecular,
pubmed-meshheading:15043153-Nitrogen,
pubmed-meshheading:15043153-Nitrogen Isotopes,
pubmed-meshheading:15043153-Nucleic Acid Conformation,
pubmed-meshheading:15043153-RNA, Catalytic
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pubmed:year |
2004
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pubmed:articleTitle |
Use of both direct and indirect 13C tags for probing nitrogen interactions in hairpin ribozyme models by 15N NMR.
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pubmed:affiliation |
Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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