Source:http://linkedlifedata.com/resource/pubmed/id/15039954
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2004-3-24
|
| pubmed:abstractText |
Sperm surface protein PH-20 expression was studied during spermatogenesis in pubertal and adult sheep, using molecular and histological methods. The effects of 24 hr of insulation raising scrotal temperatures to 39 degrees C on PH-20 expression in ejaculated sheep sperm were also determined. A 282 nt cDNA fragment of ovine PH-20 was identified in total RNA extracts of sheep testes, which exhibited 76% identity at the nucleotide level with the equivalent region of the human sequence. Ovine PH-20 mRNA and immunoreactivity were identified only in adult ram testis and not in peri-pubertal ram testis tubules lacking round spermatids, nor in adult sheep brain, pituitary, heart, spleen, lung, liver, kidney, epididymis, or ovary. Ovine PH-20 protein was distributed predominantly on the postacrosomal membrane and was also present on the anterior membrane of the sperm head in fresh, unheated sheep semen. Scrotal heating caused a significant, transient decrease in the percentage of PH-20 immunoreactive sperm, but did not change the pattern of PH-20 staining on the sperm head. The results strongly suggest that ovine PH-20 is postmeiotically expressed in haploid germ cells in sheep testis and is arrayed on the membrane of the mature ovine spermatozoon. Scrotal heating appears to have few effects on PH-20 expression and distribution on ejaculated sperm.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronoglucosaminidase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/hyaluronidase PH-20
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1040-452X
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2004 Wiley-Liss, Inc.
|
| pubmed:issnType |
Print
|
| pubmed:volume |
68
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
103-14
|
| pubmed:dateRevised |
2010-4-1
|
| pubmed:meshHeading |
pubmed-meshheading:15039954-Aging,
pubmed-meshheading:15039954-Amino Acid Sequence,
pubmed-meshheading:15039954-Animals,
pubmed-meshheading:15039954-Cell Adhesion Molecules,
pubmed-meshheading:15039954-Ejaculation,
pubmed-meshheading:15039954-Gene Expression Regulation,
pubmed-meshheading:15039954-Hot Temperature,
pubmed-meshheading:15039954-Hyaluronoglucosaminidase,
pubmed-meshheading:15039954-Immunohistochemistry,
pubmed-meshheading:15039954-In Situ Hybridization,
pubmed-meshheading:15039954-Male,
pubmed-meshheading:15039954-Molecular Sequence Data,
pubmed-meshheading:15039954-RNA, Messenger,
pubmed-meshheading:15039954-Scrotum,
pubmed-meshheading:15039954-Seminiferous Tubules,
pubmed-meshheading:15039954-Sequence Alignment,
pubmed-meshheading:15039954-Sheep,
pubmed-meshheading:15039954-Spermatogenesis,
pubmed-meshheading:15039954-Spermatozoa,
pubmed-meshheading:15039954-Testis,
pubmed-meshheading:15039954-Tissue Extracts
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Effects of scrotal heating on sperm surface protein PH-20 expression in sheep.
|
| pubmed:affiliation |
Department of Anatomy and Structural Biology, Otago School of Medical Science, University of Otago, Dunedin, New Zealand. jean.fleming@stonebow.otago.ac.nz
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|