Linked Life Data

15039558

Source:http://linkedlifedata.com/resource/pubmed/id/15039558

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 4
pubmed:dateCreated
2004-3-24
pubmed:abstractText
In this paper, the detection of crystalline elements in protein crystallization droplets containing precipitate is illustrated using the rotating-polarizer microscope technique. The sensitivity of this automated birefringence technique enables the detection of microcrystals in a precipitate that appears to be amorphous using traditional methods of inspection. The technique is illustrated with lysozyme and glucose isomerase. Glucose isomerase microcrystals were used successfully for seeding experiments and the conditions of both of the systems were refined to produce crystals suitable for X-ray analysis. The results are relevant to the field of high-throughput crystallography as an automated crystal-detection method as well as being a useful tool for detailed precipitate analysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0907-4449
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
696-702
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Assessing crystallization droplets using birefringence.
pubmed:affiliation
University of Warwick, Department of Biological Sciences, Gibbet Hill Road, Coventry CV4 7AL, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't