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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1992-9-16
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pubmed:abstractText |
Glutathione S-transferase has been purified from bovine erythrocytes by affinity chromatography. The enzyme has an isoelectric point of 7.2, behaves as a 48-kDa protein composed of two identical subunits, and has an N-terminal sequence of PPYTIVYFPVQGR?EAMRMLL. This sequence, the amino acid composition, and the kinetic parameters suggest that the enzyme belongs to the pi-class of transferases. Hemins, porphyrins, and fatty acids form complexes with the enzyme and serve as effective inhibitors. Treatment of the transferase with N-ethylmaleimide, 3-amino-1,2,4-triazole, diethyl pyrocarbonate, or 2,3-butanedione inhibits transferase activity without altering tetrapyrrole binding. The role of the complexation and inhibition of glutathione S-transferase in erythroid metabolism has yet to be elucidated.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0158-5231
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
265-74
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1503563-Amino Acid Sequence,
pubmed-meshheading:1503563-Animals,
pubmed-meshheading:1503563-Cattle,
pubmed-meshheading:1503563-Erythrocytes,
pubmed-meshheading:1503563-Fatty Acids,
pubmed-meshheading:1503563-Glutathione Transferase,
pubmed-meshheading:1503563-Hemin,
pubmed-meshheading:1503563-Kinetics,
pubmed-meshheading:1503563-Molecular Sequence Data,
pubmed-meshheading:1503563-Porphyrins,
pubmed-meshheading:1503563-Protein Binding
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pubmed:year |
1992
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pubmed:articleTitle |
Bovine erythrocyte glutathione S-transferase: purification, inhibition, and complex formation.
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pubmed:affiliation |
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0606.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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