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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2004-3-23
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pubmed:abstractText |
We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0146-9592
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
584-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15035478-3T3 Cells,
pubmed-meshheading:15035478-Animals,
pubmed-meshheading:15035478-Anisotropy,
pubmed-meshheading:15035478-Cell Physiological Phenomena,
pubmed-meshheading:15035478-Dogs,
pubmed-meshheading:15035478-Equipment Design,
pubmed-meshheading:15035478-Fluorescence,
pubmed-meshheading:15035478-Mice,
pubmed-meshheading:15035478-Microscopy, Fluorescence,
pubmed-meshheading:15035478-Microscopy, Polarization,
pubmed-meshheading:15035478-Models, Theoretical,
pubmed-meshheading:15035478-Sea Urchins,
pubmed-meshheading:15035478-Time Factors,
pubmed-meshheading:15035478-Viscosity
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pubmed:year |
2004
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pubmed:articleTitle |
Time-resolved fluorescence anisotropy imaging applied to live cells.
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pubmed:affiliation |
Departments of Physics, Biological Sciences and Chemistry, Imperial College London, London SW7 2BW, UK. k.suhling@imperial.ac.uk
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pubmed:publicationType |
Journal Article
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