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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-3-22
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pubmed:abstractText |
Activation of phospholipase D was investigated after treatment of GH3 cells with thyrotropin-releasing hormone. Thyrotropin-releasing hormone treatment resulted in both time- and dose-dependent increases of phospholipase D activity, translocation of protein kinase C-alpha and -beta I isozymes from cytosol to membrane within 30 min, and approx 43-fold increase of phosphatidylinositol-specific phospholipase C activity. Intracellular calcium concentration was rapidly increased and diacyglycerol level remained high up to 3 h after the treatment. Pretreatment of the cells with U73122, a potent inhibitor of phosphatidylinositol-specific phospholipase C, inhibited thyrotropin-releasing hormone-induced phospholipase D activation. Protein kinase C activity was down-regulated by pretreatment of the GH3 cells with either protein kinase C inhibitors (RO320432, GF109203X) or preincubation of the cells with phorbol myristrate acetate (500 nM) for 24 h. This treatment largely abolished the thyrotropin-releasing hormone-induced activation of phospholipase D, thus further confirming the involvement of protein kinase C in the activation. These results suggest that thyrotropin-releasing hormone-induced phospholipase D activation may be due to phosphatidylinositol-specific phospholipase C, and activation of protein kinase C isozymes is responsible for this stimulation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Estrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Maleimides,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrroles,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Ro 32-0432,
http://linkedlifedata.com/resource/pubmed/chemical/Thyrotropin-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/bisindolylmaleimide I
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1355-008X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33-8
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pubmed:dateRevised |
2010-6-24
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pubmed:meshHeading |
pubmed-meshheading:15034194-Animals,
pubmed-meshheading:15034194-Calcium,
pubmed-meshheading:15034194-Cell Line, Tumor,
pubmed-meshheading:15034194-Diglycerides,
pubmed-meshheading:15034194-Enzyme Activation,
pubmed-meshheading:15034194-Enzyme Inhibitors,
pubmed-meshheading:15034194-Estrenes,
pubmed-meshheading:15034194-Indoles,
pubmed-meshheading:15034194-Isoenzymes,
pubmed-meshheading:15034194-Maleimides,
pubmed-meshheading:15034194-Phospholipase D,
pubmed-meshheading:15034194-Protein Kinase C,
pubmed-meshheading:15034194-Protein Transport,
pubmed-meshheading:15034194-Pyrroles,
pubmed-meshheading:15034194-Pyrrolidinones,
pubmed-meshheading:15034194-Rats,
pubmed-meshheading:15034194-Thyrotropin-Releasing Hormone
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pubmed:year |
2004
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pubmed:articleTitle |
Thyrotropin-releasing hormone increases phospholipase D activity through stimulation of protein kinase C in GH3 cells.
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pubmed:affiliation |
Department of Internal Medicine, College of Medicine, Hanyang University, Sungdong-Gu, Seoul 133-791, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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