Source:http://linkedlifedata.com/resource/pubmed/id/15032433
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2004-3-22
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| pubmed:abstractText |
The objective was to evaluate chemical, mechanical, and sensory attributes associated with tenderness in divergent cattle breeds--Wagyu (W; n = 12), Limousin (L; n = 12) and F1-cross (WxL; n = 12)--fed two dietary treatments (0 or 6% sunflower oil (DM basis)). A randomized complete block repeated measures design in a 3 x 2 factorial arrangement of treatments was used, and effects of breed, diet, block, and associated interactions were tested. Cattle were fed barley-based diets for an average of 259 d. Twenty-four hours postmortem (PM), steaks from the longissimus muscle (LM) were sliced, vacuum-packaged, aged (1, 3, 7, 14, 28, and 56 d PM) at 2 degrees C, and frozen (-40 degrees C) until analyzed. Wagyu steaks had lower (P < 0.05) Warner-Bratzler shear force (WBSF) values than L steaks across all aging times. At 1 d PM, W steaks required slightly more (P > 0.05) force to shear than WxL or L (0.30 and 0.11 kg, respectively); however, by d 14 PM, W steaks required 0.77 kg less (P < 0.05) force to shear than L. Wagyu steaks received higher (P < 0.05) sensory panel sustained tenderness scores at d 14 PM than L. The pH decline was slower (P < 0.05), and temperature decline more (P < 0.05) rapid, in W carcasses than L or WxL carcasses. Breed and diet did not affect (P > 0.10) free calcium levels (FCL) over time (0, 1, 3, 7, and 14 d PM), 0-h calpastatin activity (CA), d-1 percent collagen (OH-PRO), or d-1 collagen cross-linking (HP). Western blot analysis for the presence of the troponin-T (TNT) 30-kDa fragment, conducted only on samples from steers fed the 0% sunflower oil diet, demonstrated more proteolysis by d 3 PM in L than W or WxL. Overall, breed differences in mechanical and sensory measures of tenderness were not explained by FCL, CA, OH-Pro, and HP. Even though the initial appearance of the TNT 30-kDa fragment was greater in L, linear slopes for appearance of TNT degradation product across aging time were greater for W and WxL (P < 0.01 and P = 0.056, respectively) than for L, suggesting that tenderness differences due to breed may have been facilitated by more-rapid proteolytic degradation over time.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Dietary Fats, Unsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Oils,
http://linkedlifedata.com/resource/pubmed/chemical/Troponin T,
http://linkedlifedata.com/resource/pubmed/chemical/calpastatin,
http://linkedlifedata.com/resource/pubmed/chemical/sunflower seed oil
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0021-8812
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| pubmed:author |
pubmed-author:BusboomJ RJR,
pubmed-author:CronrathJ DJD,
pubmed-author:DodsonM VMV,
pubmed-author:DuckettS KSK,
pubmed-author:GaskinsC TCT,
pubmed-author:Huff-LonerganEE,
pubmed-author:KuberP SPS,
pubmed-author:MarksD JDJ,
pubmed-author:McCormickR JRJ,
pubmed-author:MirZZ,
pubmed-author:RAEM VMV,
pubmed-author:ReevesJ JJJ
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| pubmed:issnType |
Print
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| pubmed:volume |
82
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
770-8
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:15032433-Animals,
pubmed-meshheading:15032433-Body Composition,
pubmed-meshheading:15032433-Calcium,
pubmed-meshheading:15032433-Calcium-Binding Proteins,
pubmed-meshheading:15032433-Cattle,
pubmed-meshheading:15032433-Collagen,
pubmed-meshheading:15032433-Consumer Satisfaction,
pubmed-meshheading:15032433-Crosses, Genetic,
pubmed-meshheading:15032433-Dietary Fats, Unsaturated,
pubmed-meshheading:15032433-Food Technology,
pubmed-meshheading:15032433-Humans,
pubmed-meshheading:15032433-Hydrogen-Ion Concentration,
pubmed-meshheading:15032433-Male,
pubmed-meshheading:15032433-Meat,
pubmed-meshheading:15032433-Muscle, Skeletal,
pubmed-meshheading:15032433-Pigmentation,
pubmed-meshheading:15032433-Plant Oils,
pubmed-meshheading:15032433-Random Allocation,
pubmed-meshheading:15032433-Temperature,
pubmed-meshheading:15032433-Time Factors,
pubmed-meshheading:15032433-Troponin T
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| pubmed:year |
2004
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| pubmed:articleTitle |
Effects of biological type and dietary fat treatment on factors associated with tenderness: I. Measurements on beef longissimus muscle.
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| pubmed:affiliation |
Washington State University, Pullman 99163-6310, USA.
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| pubmed:publicationType |
Journal Article
|