Source:http://linkedlifedata.com/resource/pubmed/id/15026306
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
2004-6-11
|
pubmed:abstractText |
Recent studies have suggested that 5'AMP-activated protein kinase (AMPK) is activated in response to metabolic stresses, such as contraction, hypoxia, and the inhibition of oxidative phosphorylation, which leads to insulin-independent glucose transport in skeletal muscle. In the present study, we hypothesized that acute oxidative stress increases the rate of glucose transport via an AMPK-mediated mechanism. When rat epitrochlearis muscles were isolated and incubated in vitro in Krebs buffer containing the oxidative agent H(2)O(2), AMPKalpha1 activity increased in a time- and dose-dependent manner, whereas AMPKalpha2 activity remained unchanged. The activation of AMPKalpha1 was associated with phosphorylation of AMPK Thr(172), suggesting that an upstream kinase is involved in the activation process. H(2)O(2)-induced AMPKalpha1 activation was blocked in the presence of the antioxidant N-acetyl-l-cysteine (NAC), and H(2)O(2) significantly increased the ratio of oxidized glutathione to glutathione (GSSG/GSH) concentrations, a sensitive marker of oxidative stress. H(2)O(2) did not cause an increase in the conventional parameters of AMPK activation, such as AMP and AMP/ATP. H(2)O(2) increased 3-O-methyl-d-glucose transport, and this increase was partially, but significantly, blocked in the presence of NAC. Results were similar when the muscles were incubated in a superoxide-generating system using hypoxanthine and xanthine oxidase. Taken together, our data suggest that acute oxidative stress activates AMPKalpha1 in skeletal muscle via an AMP-independent mechanism and leads to an increase in the rate of glucose transport, at least in part, via an AMPKalpha1-mediated mechanism.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AMP-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0193-1849
|
pubmed:author |
pubmed-author:EbiharaKenK,
pubmed-author:FushikiTohruT,
pubmed-author:HayashiTatsuyaT,
pubmed-author:HosodaKiminoriK,
pubmed-author:InoueGenG,
pubmed-author:MasuzakiHiroakiH,
pubmed-author:MiyamotoLichtL,
pubmed-author:NakanoMasakoM,
pubmed-author:NakaoKazuwaK,
pubmed-author:OtakaAkiraA,
pubmed-author:SatoKenjiK,
pubmed-author:TanakaSatsukiS,
pubmed-author:ToyodaTaroT,
pubmed-author:YonemitsuShinS
|
pubmed:issnType |
Print
|
pubmed:volume |
287
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
E166-73
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:15026306-AMP-Activated Protein Kinases,
pubmed-meshheading:15026306-Acetylcysteine,
pubmed-meshheading:15026306-Animals,
pubmed-meshheading:15026306-Dose-Response Relationship, Drug,
pubmed-meshheading:15026306-Glucose,
pubmed-meshheading:15026306-Hydrogen Peroxide,
pubmed-meshheading:15026306-Isoenzymes,
pubmed-meshheading:15026306-Male,
pubmed-meshheading:15026306-Multienzyme Complexes,
pubmed-meshheading:15026306-Muscle, Skeletal,
pubmed-meshheading:15026306-Oxidative Stress,
pubmed-meshheading:15026306-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15026306-Rats,
pubmed-meshheading:15026306-Rats, Sprague-Dawley
|
pubmed:year |
2004
|
pubmed:articleTitle |
Possible involvement of the alpha1 isoform of 5'AMP-activated protein kinase in oxidative stress-stimulated glucose transport in skeletal muscle.
|
pubmed:affiliation |
Dept. of Medicine and Clinical Science, Kyoto Univ. Graduate School of Medicine, 54 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, 606-8507, Japan.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
|