Source:http://linkedlifedata.com/resource/pubmed/id/15023542
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2004-3-16
|
| pubmed:abstractText |
Human Neural Stem Cells (hNSCs) are excellent candidates for in vitro and in vivo molecular, cellular, and developmental research, and also for ex-vivo gene transfer and cell therapy in the nervous system. However, hNSCs are mortal somatic cells, and thus invariably enter an irreversible growth arrest after a finite number of cell divisions in culture. It has been proposed that this is due to telomere shortening. Here, we show that long-term cultured (up to 4 years) v-myc perpetuated hNSC lines do preserve short but stable and homogeneous telomeres (TRF and Q-FISH determinations). hNSC lines (but not strains) express high levels of telomerase activity, which is activated by v-myc, as demonstrated here. Telomerase activity is not constitutive, becoming non-detectable after differentiation (in parallel to v-myc down-regulation). hNSC lines also maintain a stable cell cycle length, mitotic potential, differentiation and neuron generation capacity, and do not express senescence-associated beta-galactosidase over years, as studied here. These data, collectively, help to explain the immortal nature of v-myc-perpetuated hNSC lines, and to establish them as excellent research tools for basic and applied neurobiological and translational studies.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0014-4827
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
294
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
559-70
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15023542-Cell Aging,
pubmed-meshheading:15023542-Cell Culture Techniques,
pubmed-meshheading:15023542-Cell Cycle,
pubmed-meshheading:15023542-Cell Differentiation,
pubmed-meshheading:15023542-Cell Line, Transformed,
pubmed-meshheading:15023542-Cell Survival,
pubmed-meshheading:15023542-DNA,
pubmed-meshheading:15023542-DNA-Binding Proteins,
pubmed-meshheading:15023542-Down-Regulation,
pubmed-meshheading:15023542-Gene Expression Regulation, Developmental,
pubmed-meshheading:15023542-Genes, myc,
pubmed-meshheading:15023542-Humans,
pubmed-meshheading:15023542-Neurons,
pubmed-meshheading:15023542-Pluripotent Stem Cells,
pubmed-meshheading:15023542-RNA, Messenger,
pubmed-meshheading:15023542-RNA Stability,
pubmed-meshheading:15023542-Telomerase,
pubmed-meshheading:15023542-Telomere
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Long-term molecular and cellular stability of human neural stem cell lines.
|
| pubmed:affiliation |
Department of Molecular Biology, Center of Molecular Biology Severo Ochoa, Autonomous University of Madrid, 28049 Madrid, Spain.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|