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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-3-16
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pubmed:abstractText |
Interleukin-6 (IL-6) is a pleiotropic acute reactant cytokine involved in inflammatory responses. To explore the role of IL-6 in inflammation, this study examined the efficacy of exogenous IL-6 in preventing intestinal ischemia/reperfusion (I/R) injury associated with small bowel transplantation (SBTx). Syngenic orthotopic SBTx was performed in Lewis rats after 6-h graft preservation in University of Wisconsin (UW) at 4 degrees C. IL-6 mutein (IL-6m, 500 microg/kg), a recombinant molecular variant of human IL-6, was subcutaneously given to donors 2 h before harvesting (IL-6mD) or to excised grafts by luminal infusion (IL-6mG). Animal survival was 100% and 75% in IL-6mD (p<0.05 vs. control) and IL-6mG groups, respectively, compared with 64.3% in untreated controls. The severity of I/R injury (e.g. epithelial denudation, villous congestion) was reduced with IL-6m, in addition to a striking increase in re-epithelization. With IL-6m, neutrophil extravasation was markedly reduced in intestinal grafts and in remote organs (e.g. lung). IL-6m mediated anti-inflammatory effects through the inhibition of I/R-induced up-regulation of intragraft and circulating IL-1-beta, tumor necrosis factor-alpha (TNF-alpha) and IL-6. IL-6m also increased intestinal graft tissue blood flow. These results show that IL-6 is effective in protecting the intestine from cold I/R injury by maintaining graft blood flow and reducing pro-inflammatory cytokine up-regulation and neutrophil infiltration.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Chemicals,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SOCS3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/SYBR Green I,
http://linkedlifedata.com/resource/pubmed/chemical/Socs3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Suppressor of Cytokine Signaling...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1600-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
482-94
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15023140-Acute Disease,
pubmed-meshheading:15023140-Animals,
pubmed-meshheading:15023140-Blotting, Western,
pubmed-meshheading:15023140-Cytokines,
pubmed-meshheading:15023140-Immunohistochemistry,
pubmed-meshheading:15023140-Inflammation,
pubmed-meshheading:15023140-Interleukin-1,
pubmed-meshheading:15023140-Interleukin-6,
pubmed-meshheading:15023140-Intestines,
pubmed-meshheading:15023140-Lung,
pubmed-meshheading:15023140-Male,
pubmed-meshheading:15023140-Mutation,
pubmed-meshheading:15023140-Neutrophils,
pubmed-meshheading:15023140-Organ Transplantation,
pubmed-meshheading:15023140-Organic Chemicals,
pubmed-meshheading:15023140-Peroxidase,
pubmed-meshheading:15023140-RNA, Messenger,
pubmed-meshheading:15023140-Rats,
pubmed-meshheading:15023140-Rats, Inbred Lew,
pubmed-meshheading:15023140-Reperfusion Injury,
pubmed-meshheading:15023140-Repressor Proteins,
pubmed-meshheading:15023140-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15023140-Suppressor of Cytokine Signaling Proteins,
pubmed-meshheading:15023140-Time Factors,
pubmed-meshheading:15023140-Transcription Factors,
pubmed-meshheading:15023140-Tumor Necrosis Factor-alpha,
pubmed-meshheading:15023140-Up-Regulation
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pubmed:year |
2004
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pubmed:articleTitle |
Exogenous IL-6 inhibits acute inflammatory responses and prevents ischemia/reperfusion injury after intestinal transplantation.
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pubmed:affiliation |
Thomas E Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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