Linked Life Data

15020679

Source:http://linkedlifedata.com/resource/pubmed/id/15020679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 8
pubmed:dateCreated
2004-3-15
pubmed:abstractText
Accumulating reports demonstrate that apoptosis does not explain all the forms of programmed cell death (PCD), particularly in individual development and neurodegenerative disease. Recently, a novel type of PCD, designated 'paraptosis', was described. Here, we show that overexpression of TAJ/TROY, a member of the tumor necrosis factor receptor superfamily, induces non-apoptotic cell death with paraptosis-like morphology in 293T cells. Transmission electron microscopy studies reveal extensive cytoplasmic vacuolation and mitochondrial swelling in some dying cells and no condensation or fragmentation of the nuclei. Characteristically, cell death triggered by TAJ/TROY was accompanied by phosphatidylserine externalization, loss of the mitochondrial transmembrane potential and independent of caspase activation. In addition, TAJ/TROY suppressed clonogenic growth of HEK293 and HeLa cells. Interestingly, overexpression of Programmed cell death 5 (PDCD5), an apoptosis-promoting protein, enhanced TAJ/TROY-induced paraptotic cell death. Moreover, cellular endogenous PDCD5 protein was significantly upregulated in response to TAJ/TROY overexpression. These results provide novel evidence that TAJ/TROY activates a death pathway distinct from apoptosis and that PDCD5 is an important regulator in both apoptotic and non-apoptotic PCD.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1525-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15020679-Apoptosis, pubmed-meshheading:15020679-Apoptosis Regulatory Proteins, pubmed-meshheading:15020679-Cell Death, pubmed-meshheading:15020679-Cell Line, pubmed-meshheading:15020679-Cell Nucleus, pubmed-meshheading:15020679-Cells, Cultured, pubmed-meshheading:15020679-Clone Cells, pubmed-meshheading:15020679-Epithelial Cells, pubmed-meshheading:15020679-Gene Expression Regulation, Developmental, pubmed-meshheading:15020679-Gene Expression Regulation, Neoplastic, pubmed-meshheading:15020679-HeLa Cells, pubmed-meshheading:15020679-Humans, pubmed-meshheading:15020679-Membrane Potentials, pubmed-meshheading:15020679-Mitochondria, pubmed-meshheading:15020679-Neoplasm Proteins, pubmed-meshheading:15020679-Phosphatidylserines, pubmed-meshheading:15020679-Receptors, Tumor Necrosis Factor
pubmed:year
2004
pubmed:articleTitle
An alternative form of paraptosis-like cell death, triggered by TAJ/TROY and enhanced by PDCD5 overexpression.
pubmed:affiliation
Laboratory of Medical Immunology, School of Basic Medical Science, Peking University, Xueyuan Road 38, Beijing 100083, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't