Linked Life Data

15020074

Source:http://linkedlifedata.com/resource/pubmed/id/15020074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2004-3-15
pubmed:abstractText
We report on the in vivo assessment of macrophage infiltration in adoptive transfer experimental autoimmune neuritis (AT-EAN), a prototypic Th-1-mediated autoimmune disorder of the peripheral nervous system (PNS). Lewis rats received systemic injections of superparamagnetic iron oxide (SPIO) particles at days 2, 3, 4, 5, or 9 after adoptive transfer of P2-specific T-cells and were scanned by standard 1.5 T magnetic resonance imaging (MRI) always 24 h later. MRI revealed focal signal loss of the cauda equina indicating iron accumulation in spinal nerves already at the preclinical stage (day 3). Signal loss peaked at day 4, when first clinical signs developed. At the maximum of clinical disease, signal loss already declined and disappeared on days 6 and 10. In spinal nerve sections, Perl's iron stain showed focal cellular accumulation of SPIO at days 3, 4, and 5 in ED1-positive macrophages. Spinal nerves at days 6 and 10 exhibited massive macrophage infiltrates, but no more iron deposition. In conclusion, SPIO-enhanced MRI provides a novel in vivo tool to assess the timing of macrophage entry into target tissues during an immunopathologic attack and holds promise to monitor immunotherapeutic interventions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0165-5728
pubmed:author
pubmed:issnType
Print
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
142-6
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
In vivo monitoring of macrophage infiltration in experimental autoimmune neuritis by magnetic resonance imaging.
pubmed:affiliation
Department of Neurology, Julius-Maximilians Universität, Josef-Schneider-Str. 11, D-97080, Würzburg, Germany. stoll_g@klinik.uni-wuerzburg.de
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't