Linked Life Data

15019296

Source:http://linkedlifedata.com/resource/pubmed/id/15019296

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2004-3-15
pubmed:abstractText
We developed a new phagemid system for the generation of combinatorial antibody libraries. This system allows the recombination efficiency to be estimated easily, which aids the accurate measurement of the antibody library size. Two phagemids were constructed. pRTCB bears the structural tetracycline-resistant gene (tetR) and pRTCA bears its promoter region (Ptet). pRTCA and pRTCB were constructed so that recombinase-mediated chain exchange (RMCE) in Cre-expressing bacteria results in the simultaneous acquisition of Tet resistance and newly recombined single chain variable fragment (scFv) genes. PCR and restriction enzyme analysis of randomly selected tetR phagemids showed that all Tet-resistant phagemids have an active tetR gene and a recombined scFv gene. RMCE efficiency was measured by the ratio of the tetR colonies to the total colonies. The scFv proteins expressed from the recombined phagemid vectors were functional. Thus, our phagemid vector system may be useful for making combinatorial antibody libraries.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0165-2478
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
247-53
pubmed:dateRevised
2006-8-24
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
A phagemid system enabling easy estimation of the combinatorial antibody library size.
pubmed:affiliation
Department of Microbiology, School of Medicine, Ajou University, Woncheon-dong 5, Suwon 442-749, South Korea.
pubmed:publicationType
Journal Article