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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
2004-3-15
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pubmed:abstractText |
The effects of interleukine-15 (IL-15) on macrophage activation and antiviral activity have been investigated in this study. We have provided evidence that IL-15 stimulates murine macrophage RAW 264.7 cells to release nitric oxide (NO) and inhibit vaccinia virus (VV) replication in bystander human 293 cells in a dose-dependent manner. The IL-15-induced antiviral activity was partially mediated by NO, as blocking NO production by NO synthase (iNOS) inhibitor NG-monomethyl-L-arginine acetate (L-NMA) partially restored the virus replication. Interferon-gamma (IFN-gamma) was not detectable by ELISA in the cell supernatant of IL-15-activated macrophages or in the co-cultures of macrophages and infected bystander cells. Neutralizing anti-IFN-gamma, anti-IFN-gamma receptor R2, anti-TNF-alpha, or anti-IL-12 antibodies had no effect on NO production or antiviral activity. In contrast, neutralizing anti-IFN-alpha/beta antibody completely restored the VV replication and reduced the NO level to one third of that in the control. Elevated mRNA levels of IFN-beta and iNOS genes were detected in IL-15-activated RAW 264.7 cells by RT-PCR. Our data suggest that IL-15 is capable of inducing IFN-beta, which could participate in NO-mediated antiviral effect.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-15,
http://linkedlifedata.com/resource/pubmed/chemical/NOS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen Oxides,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/omega-N-Methylarginine
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0165-2478
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-8
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pubmed:dateRevised |
2011-10-27
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pubmed:meshHeading |
pubmed-meshheading:15019287-Animals,
pubmed-meshheading:15019287-Bystander Effect,
pubmed-meshheading:15019287-Cell Line,
pubmed-meshheading:15019287-Gene Expression Regulation,
pubmed-meshheading:15019287-Humans,
pubmed-meshheading:15019287-Interferon-beta,
pubmed-meshheading:15019287-Interferon-gamma,
pubmed-meshheading:15019287-Interleukin-15,
pubmed-meshheading:15019287-Macrophages,
pubmed-meshheading:15019287-Mice,
pubmed-meshheading:15019287-Nitric Oxide Synthase,
pubmed-meshheading:15019287-Nitric Oxide Synthase Type II,
pubmed-meshheading:15019287-Nitrogen Oxides,
pubmed-meshheading:15019287-RNA, Messenger,
pubmed-meshheading:15019287-Transcription, Genetic,
pubmed-meshheading:15019287-Tumor Necrosis Factor-alpha,
pubmed-meshheading:15019287-Vaccinia virus,
pubmed-meshheading:15019287-Virus Replication,
pubmed-meshheading:15019287-omega-N-Methylarginine
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pubmed:year |
2004
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pubmed:articleTitle |
IL-15 induces IFN-beta and iNOS gene expression, and antiviral activity of murine macrophage RAW 264.7 cells.
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pubmed:affiliation |
Advanced Biosystems Inc., George Mason University, 10900 University Boulevard, MSN 1A8, Manassas, VA 20110, USA. ge.liu@analex.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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