Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-3-10
pubmed:abstractText
Dihydropyridine (DHP) type Ca2+ channel blocker (CCB) is effective in treatment of hepatitis in man. L-type Ca2+ channel is a target of DHP-CCB, and basic studies suggest that L-type Ca2+ channel alpha1D-subunit (Cav 1.3) seems to be a target of drug development for the treatment of hepatitis. Mouse hepatitis model is useful to study the effect of DHP-CCB on hepatitis. In order to use mouse hepatitis model to screen DHP-CCB specific for Cav 1.3, Cav 1.3 expressed in the mouse liver should have enough structural homology with that of human Cav 1.3. cDNA of the Cav 1.3 was cloned from mouse brain by reverse transcription polymerase chain reaction. The primary structure of the mouse Cav 1.3 comprises an open reading frame of 6540 bp encoding 2180 amino acids. Liver transcript lacked 60 and 45 bases from 1497 to 1556, and from 3949 to 3993 of the sequence, respectively, due to results of an alternative splicing. The present results indicated that mouse Cav 1.3 exhibited 96% homology with human Cav 1.3 and was expressed in the liver. Thus, mouse hepatitis model seemed to be useful to screen DHP-CCB specific for Cav 1.3.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1107-3756
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
573-6
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed-meshheading:15010858-Alternative Splicing, pubmed-meshheading:15010858-Animals, pubmed-meshheading:15010858-Brain, pubmed-meshheading:15010858-Calcium Channel Blockers, pubmed-meshheading:15010858-Calcium Channels, pubmed-meshheading:15010858-Calcium Channels, L-Type, pubmed-meshheading:15010858-Cloning, Molecular, pubmed-meshheading:15010858-DNA, Complementary, pubmed-meshheading:15010858-Dihydropyridines, pubmed-meshheading:15010858-Female, pubmed-meshheading:15010858-Humans, pubmed-meshheading:15010858-Liver, pubmed-meshheading:15010858-Mice, pubmed-meshheading:15010858-Mice, Inbred BALB C, pubmed-meshheading:15010858-Models, Genetic, pubmed-meshheading:15010858-Open Reading Frames, pubmed-meshheading:15010858-Polymerase Chain Reaction, pubmed-meshheading:15010858-RNA, pubmed-meshheading:15010858-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2004
pubmed:articleTitle
Cloning of the Cav 1.3 (alpha1D) L-type Ca2+ channel from mouse and its expression in the liver.
pubmed:affiliation
Research Laboratories, Nippon Chemiphar Co., Ltd., Saitama 341-0005, Japan.
pubmed:publicationType
Journal Article