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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-9-17
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pubmed:abstractText |
A high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 278 nm is presented for the determination of 5,10-dideaza-5,6,7,8-tetrahydrofolic acid in plasma. Sample pretreatment was achieved by using cation-exchange solid-phase extraction columns with methotrexate as internal standard. Chromatographic separation was based on ion-pair HPLC with 1-octanesulphonic acid as the ion-pairing compound. The detection limit was 10 ng/ml using an 500-microliters sample volume. The assay was linear from the detection limit up to 5000 ng/ml with good reproducibility. The applicability of the assay was demonstrated in a study in the rat.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9673
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
576
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
158-62
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:1500451-Animals,
pubmed-meshheading:1500451-Antineoplastic Agents,
pubmed-meshheading:1500451-Chromatography, High Pressure Liquid,
pubmed-meshheading:1500451-Folic Acid Antagonists,
pubmed-meshheading:1500451-Indicators and Reagents,
pubmed-meshheading:1500451-Male,
pubmed-meshheading:1500451-Rats,
pubmed-meshheading:1500451-Rats, Inbred Strains,
pubmed-meshheading:1500451-Spectrophotometry, Ultraviolet,
pubmed-meshheading:1500451-Tetrahydrofolates
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pubmed:year |
1992
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pubmed:articleTitle |
Bioanalysis of the investigational anti-tumour drug 5,10-dideaza-5,6,7,8-tetrahydrofolic acid by high-performance liquid chromatography with ultraviolet detection.
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pubmed:affiliation |
Department of Clinical Chemistry, Netherlands Cancer Institute, Amsterdam.
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pubmed:publicationType |
Journal Article
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