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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1992-9-16
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pubmed:abstractText |
Glucosylceramide (Glc beta 1-1Cer) and a novel ceramide trihexoside (Gal beta 1-6Gal beta 1-6Glc beta 1-1Cer) were purified from the eggs of the sea urchin, Hemicentrotus pulcherrimus. Their chemical structures were determined by gas-liquid chromatography, methylation analysis, chromic acid oxidation, enzymatic hydrolysis, enzyme-linked immunosorbent assay, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance spectroscopy. The ceramide trihexoside has a novel carbohydrate structure, and its core structure, Gal beta 1-6Glc, is also novel. The ceramide moieties of these glycolipids are almost identical. Two fatty acids, 22:1 and 22h:1, constitute more than 80% of the total acids. Long-chain bases are all phytosphingosine, approximately 90% of which is n-t18:0. The finding of melibiosylceramide (Gal alpha 1-6Glc beta 1-1Cer) from the eggs of another sea urchin species [Kubo, H. et al. (1988) J. Biochem. 104, 755-760] and the present finding of the novel ceramide trihexoside suggest that there are a variety of unique sugar structures in sea urchin glycosphingolipids.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-924X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
111
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
726-31
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:1500414-Animals,
pubmed-meshheading:1500414-Carbohydrate Conformation,
pubmed-meshheading:1500414-Carbohydrate Sequence,
pubmed-meshheading:1500414-Fatty Acids,
pubmed-meshheading:1500414-Female,
pubmed-meshheading:1500414-Magnetic Resonance Spectroscopy,
pubmed-meshheading:1500414-Molecular Sequence Data,
pubmed-meshheading:1500414-Ovum,
pubmed-meshheading:1500414-Sea Urchins,
pubmed-meshheading:1500414-Spectrometry, Mass, Fast Atom Bombardment,
pubmed-meshheading:1500414-Trihexosylceramides
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pubmed:year |
1992
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pubmed:articleTitle |
A novel ceramide trihexoside from the eggs of the sea urchin, Hemicentrotus pulcherrimus.
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pubmed:affiliation |
Department of Life Science, Tokyo Institute of Technology, Kanagawa.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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