Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-3-8
pubmed:abstractText
The objective of this study was to analyze the changes in the type 1 T cell response, including the CD4+ Th1 and CD8+ T cell responses, to influenza in the elderly compared with those in young adults. PBMC activated ex vivo with influenza virus exhibited an age-related decline in type 1 T cell response, shown by the decline in the frequency of IFN-gamma-secreting memory T cells specific for influenza (IFN-gamma+ ISMT) using ELISPOT or intracellular cytokine staining. The reduced frequency of IFN-gamma+ ISMT was accompanied by a reduced level of IFN-gamma secretion per cell in elderly subjects. Tetramer staining, combined with IFN-gamma ELISPOT, indicated that the decline in IFN-gamma+, influenza M1-peptide-specific T cells was not due to attrition of the T cell repertoire, but, rather, to the functional loss of ISMT with age. In addition, the decline in type 1 T cell response was not due to an increase in Th2 response or defects in APCs from the elderly. The expansion of influenza-specific CD8+ T cells in CTL cultures was reduced in the elderly. Compared with young subjects, frail elderly subjects also exhibited a blunted and somewhat delayed type 1 T cell response to influenza vaccination, which correlated positively with the reduced IgG1 subtype and the total Ab response. Taken together, these data demonstrate that there is a decline in the type 1 T cell response to influenza with age that may help explain the age-related decline in vaccine efficacy and the increases in influenza morbidity and mortality.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3437-46
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15004143-Adult, pubmed-meshheading:15004143-Aged, pubmed-meshheading:15004143-Aged, 80 and over, pubmed-meshheading:15004143-Aging, pubmed-meshheading:15004143-Antibodies, Viral, pubmed-meshheading:15004143-Cell Division, pubmed-meshheading:15004143-Cells, Cultured, pubmed-meshheading:15004143-Cytokines, pubmed-meshheading:15004143-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:15004143-Epitopes, T-Lymphocyte, pubmed-meshheading:15004143-Flow Cytometry, pubmed-meshheading:15004143-Frail Elderly, pubmed-meshheading:15004143-Herpesvirus 3, Human, pubmed-meshheading:15004143-Humans, pubmed-meshheading:15004143-Influenza A virus, pubmed-meshheading:15004143-Influenza Vaccines, pubmed-meshheading:15004143-Interferon-gamma, pubmed-meshheading:15004143-Leukocytes, Mononuclear, pubmed-meshheading:15004143-Lymphocyte Activation, pubmed-meshheading:15004143-Middle Aged, pubmed-meshheading:15004143-Th1 Cells, pubmed-meshheading:15004143-Th2 Cells, pubmed-meshheading:15004143-Viral Matrix Proteins
pubmed:year
2004
pubmed:articleTitle
Age-related impaired type 1 T cell responses to influenza: reduced activation ex vivo, decreased expansion in CTL culture in vitro, and blunted response to influenza vaccination in vivo in the elderly.
pubmed:affiliation
Glennan Center for Geriatrics and Gerontology, Department of Internal Medicine, Eastern Virginia Medical School, Norfolk, VA 23507, USA. dengy@evms.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't