rdf:type |
|
lifeskim:mentions |
umls-concept:C0001792,
umls-concept:C0010453,
umls-concept:C0021400,
umls-concept:C0039194,
umls-concept:C0039195,
umls-concept:C0042200,
umls-concept:C0205216,
umls-concept:C0221099,
umls-concept:C0332307,
umls-concept:C0392756,
umls-concept:C0871261,
umls-concept:C1515655,
umls-concept:C1533691,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1879547,
umls-concept:C2348480,
umls-concept:C2911692
|
pubmed:issue |
6
|
pubmed:dateCreated |
2004-3-8
|
pubmed:abstractText |
The objective of this study was to analyze the changes in the type 1 T cell response, including the CD4+ Th1 and CD8+ T cell responses, to influenza in the elderly compared with those in young adults. PBMC activated ex vivo with influenza virus exhibited an age-related decline in type 1 T cell response, shown by the decline in the frequency of IFN-gamma-secreting memory T cells specific for influenza (IFN-gamma+ ISMT) using ELISPOT or intracellular cytokine staining. The reduced frequency of IFN-gamma+ ISMT was accompanied by a reduced level of IFN-gamma secretion per cell in elderly subjects. Tetramer staining, combined with IFN-gamma ELISPOT, indicated that the decline in IFN-gamma+, influenza M1-peptide-specific T cells was not due to attrition of the T cell repertoire, but, rather, to the functional loss of ISMT with age. In addition, the decline in type 1 T cell response was not due to an increase in Th2 response or defects in APCs from the elderly. The expansion of influenza-specific CD8+ T cells in CTL cultures was reduced in the elderly. Compared with young subjects, frail elderly subjects also exhibited a blunted and somewhat delayed type 1 T cell response to influenza vaccination, which correlated positively with the reduced IgG1 subtype and the total Ab response. Taken together, these data demonstrate that there is a decline in the type 1 T cell response to influenza with age that may help explain the age-related decline in vaccine efficacy and the increases in influenza morbidity and mortality.
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pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Influenza Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/M-protein, influenza virus,
http://linkedlifedata.com/resource/pubmed/chemical/M1 protein, Influenza A virus,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
|
pubmed:issn |
0022-1767
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
15
|
pubmed:volume |
172
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
3437-46
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15004143-Adult,
pubmed-meshheading:15004143-Aged,
pubmed-meshheading:15004143-Aged, 80 and over,
pubmed-meshheading:15004143-Aging,
pubmed-meshheading:15004143-Antibodies, Viral,
pubmed-meshheading:15004143-Cell Division,
pubmed-meshheading:15004143-Cells, Cultured,
pubmed-meshheading:15004143-Cytokines,
pubmed-meshheading:15004143-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15004143-Epitopes, T-Lymphocyte,
pubmed-meshheading:15004143-Flow Cytometry,
pubmed-meshheading:15004143-Frail Elderly,
pubmed-meshheading:15004143-Herpesvirus 3, Human,
pubmed-meshheading:15004143-Humans,
pubmed-meshheading:15004143-Influenza A virus,
pubmed-meshheading:15004143-Influenza Vaccines,
pubmed-meshheading:15004143-Interferon-gamma,
pubmed-meshheading:15004143-Leukocytes, Mononuclear,
pubmed-meshheading:15004143-Lymphocyte Activation,
pubmed-meshheading:15004143-Middle Aged,
pubmed-meshheading:15004143-Th1 Cells,
pubmed-meshheading:15004143-Th2 Cells,
pubmed-meshheading:15004143-Viral Matrix Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Age-related impaired type 1 T cell responses to influenza: reduced activation ex vivo, decreased expansion in CTL culture in vitro, and blunted response to influenza vaccination in vivo in the elderly.
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pubmed:affiliation |
Glennan Center for Geriatrics and Gerontology, Department of Internal Medicine, Eastern Virginia Medical School, Norfolk, VA 23507, USA. dengy@evms.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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