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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-4-5
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pubmed:abstractText |
To establish a more efficient treatment for immunotherapy against solid tumors, we have evaluated the antitumor effect by coexpression of a chemokine CCL21/secondary lymphoid tissue chemokine and a costimulatory molecule LIGHT in colon carcinoma C26. C26 cells expressing either CCL21 or LIGHT exhibited a significantly reduced tumor growth in vivo, and mice inoculated with these cells showed a prolonged survival, but eventually all these mice died. In contrast, C26 cells expressing both CCL21 and LIGHT exhibited a minimal tumor growth in vivo, and all these mice survived healthily with a tumor remission and consequently acquired a strong protective immunity. A markedly increased infiltration of mature dendritic cells (DCs), and CD8(+) T cells was observed in the tumor mass, and their spleen cells showed a greatly enhanced cytotoxic T lymphocyte (CTL) activity against C26 tumor and interferon (IFN)-gamma production. Neutralization of IFN-gamma or depletion of CD8(+) or CD4(+) T cells significantly reduced the antitumor activity. These results suggest that the combined treatment with CCL21 and LIGHT is able to induce a synergistic antitumor effect to eradicate tumor completely by greatly enhancing tumor-infiltration of lymphocytes including mature DCs and CD8(+) T cells, resulting in markedly augmented CTL activity and IFN-gamma production.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCL21 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ccl21c protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL21,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/TNFSF14 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfsf14 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor Ligand...,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0929-1903
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
280-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15002032-Animals,
pubmed-meshheading:15002032-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15002032-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15002032-Carcinoma,
pubmed-meshheading:15002032-Cell Line, Tumor,
pubmed-meshheading:15002032-Chemokine CCL21,
pubmed-meshheading:15002032-Chemokines, CC,
pubmed-meshheading:15002032-Chemotaxis, Leukocyte,
pubmed-meshheading:15002032-Colonic Neoplasms,
pubmed-meshheading:15002032-Dendritic Cells,
pubmed-meshheading:15002032-Female,
pubmed-meshheading:15002032-Gene Expression,
pubmed-meshheading:15002032-Humans,
pubmed-meshheading:15002032-Interferon-gamma,
pubmed-meshheading:15002032-Membrane Proteins,
pubmed-meshheading:15002032-Mice,
pubmed-meshheading:15002032-Mice, Inbred BALB C,
pubmed-meshheading:15002032-Neoplasm Transplantation,
pubmed-meshheading:15002032-Neoplasms, Experimental,
pubmed-meshheading:15002032-RNA, Messenger,
pubmed-meshheading:15002032-Survival Rate,
pubmed-meshheading:15002032-Transfection,
pubmed-meshheading:15002032-Tumor Necrosis Factor Ligand Superfamily Member 14,
pubmed-meshheading:15002032-Tumor Necrosis Factor-alpha
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pubmed:year |
2004
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pubmed:articleTitle |
Synergistic antitumor effect by coexpression of chemokine CCL21/SLC and costimulatory molecule LIGHT.
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pubmed:affiliation |
Intractable Immune System Disease Research Center, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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