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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5667
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pubmed:dateCreated |
2004-4-6
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pubmed:abstractText |
We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion base pairs of nonredundant sequence was generated, annotated, and analyzed to elucidate the gene content, diversity, and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 previously unknown bacterial phylotypes. We have identified over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1095-9203
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pubmed:author |
pubmed-author:Baden-TillsonHollyH,
pubmed-author:EisenJonathan AJA,
pubmed-author:FoutsDerrick EDE,
pubmed-author:HalpernAaron LAL,
pubmed-author:HeidelbergJohn FJF,
pubmed-author:HoffmanJeffJ,
pubmed-author:KnapAnthony HAH,
pubmed-author:LevySamuelS,
pubmed-author:LomasMichael WMW,
pubmed-author:NealsonKenK,
pubmed-author:NelsonKaren EKE,
pubmed-author:NelsonWilliamW,
pubmed-author:ParsonsRachelR,
pubmed-author:PaulsenIanI,
pubmed-author:PetersonJeremyJ,
pubmed-author:PfannkochCynthiaC,
pubmed-author:RemingtonKarinK,
pubmed-author:RogersYu-HuiYH,
pubmed-author:RuschDougD,
pubmed-author:SmithHamilton OHO,
pubmed-author:VenterJ CraigJC,
pubmed-author:WhiteOwenO,
pubmed-author:WuDongyingD
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pubmed:issnType |
Electronic
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pubmed:day |
2
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pubmed:volume |
304
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
66-74
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:15001713-Archaea,
pubmed-meshheading:15001713-Atlantic Ocean,
pubmed-meshheading:15001713-Bacteria,
pubmed-meshheading:15001713-Bacteriophages,
pubmed-meshheading:15001713-Biodiversity,
pubmed-meshheading:15001713-Computational Biology,
pubmed-meshheading:15001713-Cyanobacteria,
pubmed-meshheading:15001713-Ecosystem,
pubmed-meshheading:15001713-Eukaryotic Cells,
pubmed-meshheading:15001713-Genes, Archaeal,
pubmed-meshheading:15001713-Genes, Bacterial,
pubmed-meshheading:15001713-Genes, rRNA,
pubmed-meshheading:15001713-Genome, Archaeal,
pubmed-meshheading:15001713-Genome, Bacterial,
pubmed-meshheading:15001713-Genomics,
pubmed-meshheading:15001713-Molecular Sequence Data,
pubmed-meshheading:15001713-Photosynthesis,
pubmed-meshheading:15001713-Phylogeny,
pubmed-meshheading:15001713-Plasmids,
pubmed-meshheading:15001713-Rhodopsin,
pubmed-meshheading:15001713-Seawater,
pubmed-meshheading:15001713-Sequence Analysis, DNA,
pubmed-meshheading:15001713-Water Microbiology
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pubmed:year |
2004
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pubmed:articleTitle |
Environmental genome shotgun sequencing of the Sargasso Sea.
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pubmed:affiliation |
Institute for Biological Energy Alternatives, 1901 Research Boulevard, Rockville, MD 20850, USA. jcventer@tcag.org
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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