Source:http://linkedlifedata.com/resource/pubmed/id/14998555
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2004-3-4
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| pubmed:abstractText |
The present study investigates the role of nitric oxide (NO) on restraint stress (RS)-induced modulation of humoral and cell-mediated immune responses in rats and mice. RS produced suppression of humoral immune response, i.e., anti-SRBC antibody titre ( 7.38 +/- 0.32 versus 4.13 +/- 0.30; mean +/- S.E.M., P < 0.001). In case of cell-mediated immunity, in delayed type hypersensitivity (DTH) response the change in paw volume decreased from 0.069 +/- 0.003 mm (mean +/- S.E.M.) in control non-stressed group to 0.038 +/- 0.002 mm in the stressed group (P < 0.001) while percentage leucocyte migration inhibition (% LMI) decreased from 39.7 +/- 1.95 in control non-stressed animals to 15.2 +/- 1.07 in animals subjected to stress (P < 0.01). Pretreating the animals with an NO precursor, L-arginine (1000 mg kg-1, i.p.) antagonized the effect of RS on humoral (anti-SRBC antibody titre 6.50 +/- 0.27 versus 4.13 +/- 0.30, P < 0.001 ) and cell-mediated (DTH response 0.066 +/- 0.002 mm versus 0.038 +/- 0.002 mm, P < 0.001; % LMI 41.5 +/- 1.46 versus 15.2 +/- 1.07, P < 0.01) immune responses. Administration of 7-nitroindazole (7-NI, 50 mg kg-1, i.p.), an inhibitor of neuronal NO synthase, alone further enhanced the immunosuppressive effect of RS (anti-SRBC antibody titre 2.75 +/- 0.25 versus 4.13 +/- 0.30, P < 0.001; DTH response 0.019 +/- 0.002 mm versus 0.038 +/- 0.002 mm, P < 0.001; % LMI 5.0 +/- 1.08 versus 15.2 +/- 1.07, P < 0.01). However, when given before L-arginine treatment, 7-NI reversed the effect of the latter drug on stress-induced immunomodulation (anti-SRBC antibody titre 3.00 +/- 0.27 versus 6.5 +/- 0.27, P < 0.001; DTH response 0.043 +/- 0.003 mm versus 0.066 +/- 0.002 mm, P < 0.001; % LMI 12.0 +/- 0.93 versus 41.5 +/- 1.46, P < 0.01). Unlike its effect on RS-induced immune responsiveness, L-arginine (250, 500, 1000 mg kg-1) when given for 5-7 days to naive non-stressed animals produced dose dependent suppression of both humoral (anti-SRBC antibody titre 6.4 +/- 0.32 versus 5.4 +/- 0.32, 4.0 +/- 0.27, 3.1 +/- 0.30, respectively) and cell-mediated (DTH 0.065 +/- 0.003 mm versus 0.064 +/- 0.004 mm, 0.039 +/- 0.003 mm, 0.020 +/- 0.002 mm, respectively and % LMI 37.52 +/- 1.58 versus 30.48 +/- 1.07, 28.18 +/- 1.22, 19.76 +/- 0.83, respectively) immune responses. 7-NI significantly blocked these immunosuppressive effects of L-arginine (anti-SRBC antibody titre 6.0 +/- 0.38 versus 3.1 +/- 0.030, P < 0.01; DTH response 0.056 +/- 0.004 mm versus 0.020 +/- 0.002 mm, P < 0.001; % LMI 34.76 +/- 1.31 versus 19.76 +/- 0.83, P < 0.01). However, 7-NI when given to non-stressed animals failed to modulate immune responsiveness. Thus, NO appears to play an important role in RS-induced immunomodulation and these effects are different from its effect on immune responsiveness in non-stressed animals.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/7-nitroindazole,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Indazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Nos1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nos1 protein, rat
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1043-6618
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
49
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
455-60
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:14998555-Animals,
pubmed-meshheading:14998555-Antibody Formation,
pubmed-meshheading:14998555-Arginine,
pubmed-meshheading:14998555-Cell Migration Inhibition,
pubmed-meshheading:14998555-Dose-Response Relationship, Immunologic,
pubmed-meshheading:14998555-Drug Administration Schedule,
pubmed-meshheading:14998555-Erythrocytes,
pubmed-meshheading:14998555-Hypersensitivity, Delayed,
pubmed-meshheading:14998555-Immunity, Cellular,
pubmed-meshheading:14998555-Indazoles,
pubmed-meshheading:14998555-Injections, Intraperitoneal,
pubmed-meshheading:14998555-Male,
pubmed-meshheading:14998555-Mice,
pubmed-meshheading:14998555-Nerve Tissue Proteins,
pubmed-meshheading:14998555-Nitric Oxide Synthase,
pubmed-meshheading:14998555-Nitric Oxide Synthase Type I,
pubmed-meshheading:14998555-Rats,
pubmed-meshheading:14998555-Rats, Wistar,
pubmed-meshheading:14998555-Restraint, Physical,
pubmed-meshheading:14998555-Sheep,
pubmed-meshheading:14998555-Stress, Physiological,
pubmed-meshheading:14998555-Time Factors
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| pubmed:year |
2004
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| pubmed:articleTitle |
Effect of L-arginine on restraint stress induced modulation of immune responses in rats and mice.
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| pubmed:affiliation |
Department of Pharmacology, University College of Medical Sciences and GTB Hospital, New Delhi 110095, India. drkksharma2000@yahoo.com
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| pubmed:publicationType |
Journal Article,
Comparative Study
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