Source:http://linkedlifedata.com/resource/pubmed/id/14997500
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2004-3-3
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| pubmed:abstractText |
Two-dimensional difference gel electrophoresis (2-D DIGE) coupled with mass spectrometry (MS) was used to investigate tumor-specific changes in the proteome of human colorectal cancers and adjacent normal mucosa. For each of six patients with different stages of colon cancer, Cy5-labeled proteins isolated from tumor tissue were combined with Cy3-labeled proteins isolated from neighboring normal mucosa and separated on the same 2-D gel along with a Cy2-labeled mixture of all 12 normal/tumor samples as an internal standard. Over 1500 protein spot-features were analyzed in each paired normal/tumor comparison, and using DIGE technology with the mixed-sample internal standard, statistically significant quantitative comparisons of each protein abundance change could be made across multiple samples simultaneously without interference due to gel-to-gel variation. Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) and tandem (TOF/TOF) MS provided sensitive and accurate mass spectral data for database interrogation, resulting in the identification of 52 unique proteins (including redundancies due to proteolysis and post-translationally modified isoforms) that were changing in abundance across the cohort. Without the benefit of the Cy2-labeled 12 sample mixture internal standard, 42 of these proteins would have been overlooked due to the large degree of variation inherent between normal and tumor samples.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/KRT3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Keratin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Keratins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Proteome
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
1615-9853
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
4
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
793-811
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14997500-Adult,
pubmed-meshheading:14997500-Aged,
pubmed-meshheading:14997500-Aged, 80 and over,
pubmed-meshheading:14997500-Colonic Neoplasms,
pubmed-meshheading:14997500-Databases as Topic,
pubmed-meshheading:14997500-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:14997500-Female,
pubmed-meshheading:14997500-Humans,
pubmed-meshheading:14997500-Image Processing, Computer-Assisted,
pubmed-meshheading:14997500-Intestinal Mucosa,
pubmed-meshheading:14997500-Keratin-3,
pubmed-meshheading:14997500-Keratins,
pubmed-meshheading:14997500-Male,
pubmed-meshheading:14997500-Mass Spectrometry,
pubmed-meshheading:14997500-Middle Aged,
pubmed-meshheading:14997500-Protein Isoforms,
pubmed-meshheading:14997500-Proteome,
pubmed-meshheading:14997500-Proteomics,
pubmed-meshheading:14997500-Sensitivity and Specificity,
pubmed-meshheading:14997500-Spectrometry, Mass, Matrix-Assisted Laser...
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| pubmed:year |
2004
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| pubmed:articleTitle |
Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry.
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| pubmed:affiliation |
Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University, Nashville, TN 37232-8575, USA. david.friedman@vanderbilt.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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