Linked Life Data

14991355

Source:http://linkedlifedata.com/resource/pubmed/id/14991355

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-3-1
pubmed:abstractText
Patients with paraneoplastic encephalomyelitis(subacute sensory neuronopathy) (PEM/SSN), most commonly associated with small-cell lung cancer (SCLC), frequently harbor Hu antibodies, which are usually detected by indirect immunohistochemistry or immunoblot. We developed a new radioimmunobinding assay to detect Hu antibodies based on in vitro transcribed and translated (ITT) HuD. High levels of Hu antibodies were detected in all seven PEM/SSN patients tested, but not in any of 15 patients with other paraneoplastic syndromes, 20 patients with Sjögren's syndrome, 20 patients with miscellaneous tumors and 99 of 100 blood donors. One of the blood donors had low levels of Hu antibodies. The radiobinding assay detected Hu antibodies in 45 of 191 (24%) patients with SCLC, in comparison with immunofluorescence and immuno dot blot, where only 34 of 191 (18%) were detected. All patients with Hu antibodies detected by immunofluorescence and immuno dot-blot were also positive by the radioimmunobinding assay. The results demonstrate that this assay is very specific and sensitive for the detection of Hu antibodies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0340-5354
pubmed:author
pubmed:issnType
Print
pubmed:volume
251
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-203
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14991355-Antibodies, pubmed-meshheading:14991355-Female, pubmed-meshheading:14991355-Fluorescent Antibody Technique, pubmed-meshheading:14991355-Hu Paraneoplastic Encephalomyelitis Antigens, pubmed-meshheading:14991355-Humans, pubmed-meshheading:14991355-Immunoblotting, pubmed-meshheading:14991355-Male, pubmed-meshheading:14991355-Nerve Tissue Proteins, pubmed-meshheading:14991355-Neurons, Afferent, pubmed-meshheading:14991355-Paraneoplastic Syndromes, Nervous System, pubmed-meshheading:14991355-Peripheral Nervous System Diseases, pubmed-meshheading:14991355-Predictive Value of Tests, pubmed-meshheading:14991355-RNA-Binding Proteins, pubmed-meshheading:14991355-Radioimmunoassay, pubmed-meshheading:14991355-Reference Values, pubmed-meshheading:14991355-Reproducibility of Results, pubmed-meshheading:14991355-Sjogren's Syndrome
pubmed:year
2004
pubmed:articleTitle
Paraneoplastic antibodies against HuD detected by a sensitive radiobinding assay.
pubmed:affiliation
Dept. of Neurology, Haukeland University Hospital, 5021, Bergen, Norway. anette.storstein@helse-bergen.no
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't