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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2004-3-1
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pubmed:abstractText |
In this study, reverse transcriptase (RT)- and integrase (IN)-defective human immunodeficiency virus type 1 (HIV-1) was transcomplemented with Vpr-RT-IN fusion proteins to delineate pol sequences important for HIV-1 replication. Our results reveal that a 194-bp sequence encompassing the 3'end of the IN gene and containing the central DNA flap is necessary and sufficient for efficient HIV-1 single-cycle replication in dividing and nondividing cells. Furthermore, we show that the central DNA flap enhances HIV-1 single-round replication by five- to sevenfold, primarily by facilitating nuclear import of proviral DNA. In agreement with previous reports, our data support a functional role of the central DNA flap during the early stages of HIV-1 infection.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-10490769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-10786833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-10835641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-10860744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-10888652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-11110680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-11313790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-11329014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-11389849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-11502522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-11545606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-11691994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-12091904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-12228227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-12237436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-12414949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-12414950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-12663775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-12796494,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-1560526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-1720811,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-9275210,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-9371822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-9436978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-9557700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-9562972,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-9621063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990738-9837715
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, pol,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, vpr,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Integrase,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Reverse Transcriptase,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/vpr Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3170-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14990738-Cell Nucleus,
pubmed-meshheading:14990738-DNA, Viral,
pubmed-meshheading:14990738-Gene Products, pol,
pubmed-meshheading:14990738-Gene Products, vpr,
pubmed-meshheading:14990738-Genetic Complementation Test,
pubmed-meshheading:14990738-HIV Integrase,
pubmed-meshheading:14990738-HIV Reverse Transcriptase,
pubmed-meshheading:14990738-HIV-1,
pubmed-meshheading:14990738-Humans,
pubmed-meshheading:14990738-Leukocytes, Mononuclear,
pubmed-meshheading:14990738-Recombinant Fusion Proteins,
pubmed-meshheading:14990738-Virus Integration,
pubmed-meshheading:14990738-Virus Replication,
pubmed-meshheading:14990738-vpr Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2004
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pubmed:articleTitle |
Assessment of the role of the central DNA flap in human immunodeficiency virus type 1 replication by using a single-cycle replication system.
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pubmed:affiliation |
Laboratoire de rétrovirologie humaine, Département de microbiologie et immunologie, Faculté de médecine, Université de Montréal, Montréal, Québec, Canada H3C 3J7.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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