Linked Life Data

14990485

Source:http://linkedlifedata.com/resource/pubmed/id/14990485

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-3-1
pubmed:databankReference
pubmed:abstractText
Enkephalins are pentapeptides found in the central nervous system. It is believed that these neuropeptides interact with the nerve cell membrane to adopt a conformation suitable for their binding to an opiate receptor. In this work, we have determined the three-dimensional structure of methionine-enkephalin (Menk) in fast-tumbling bicelles using multidimensional (1)H NMR. Bicelles were selected as model membranes because both their bilayer organization and composition resemble those of natural biomembranes. The effect of the membrane composition on the peptide conformation was explored using both zwitterionic (PC bicelles) and negatively charged bicelles (Bic/PG). Pulsed field gradient experiments allowed the determination of the proportion of Menk bound to the model membranes. Approximately 60% of the water-soluble enkephalin was found to associate to the bicellar systems. Structure calculations from torsion angle and NOE-based distance constraints suggest the presence of both micro - and delta-selective conformers of Menk in each system and slightly different conformers in PC bicelles and Bic/PG. As opposed to previous studies of enkephalins in membrane mimetic systems, our results show that these opiate peptides could adopt several conformations in a membrane environment, which is consistent with the flexibility and poor selectivity of enkephalins.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-10423434, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-10685340, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-10797229, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-10797231, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-11159401, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-11285556, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-11470082, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-11566787, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-11683621, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-11734036, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-11916839, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-1207728, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-12495026, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-12585494, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-12829487, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-1390677, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-1420972, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-1472657, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-1654306, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-1782355, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-2157483, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-2209598, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-2554969, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-2720108, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-2835652, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-2874556, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-3024707, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-3337714, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-3458181, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-3584096, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-3801586, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-38962, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-451597, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-6243479, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-6320173, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-6501322, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-6625595, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-7756980, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-8384662, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-8520220, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-8541444, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-8589255, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-8744573, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-8856052, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-8956077, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-9109645, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-9129806, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-9229505, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-9414244, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-9545049, http://linkedlifedata.com/resource/pubmed/commentcorrection/14990485-9878482
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1587-600
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
More...