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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0013935,
umls-concept:C0016315,
umls-concept:C0019564,
umls-concept:C0027882,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035647,
umls-concept:C0178719,
umls-concept:C0182258,
umls-concept:C0185125,
umls-concept:C0220908,
umls-concept:C0242485,
umls-concept:C0596235
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pubmed:issue |
2
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pubmed:dateCreated |
2004-3-1
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pubmed:abstractText |
Fluorescence microplate readers for the measurement of cytosolic free Ca(2+) ([Ca(2+)](i)) are used as a drug screening tool, particularly for immortal cell lines. However, wider application of this methodological approach to more differentiated cells such as neurons would also be useful for the screening of compounds that modulate synaptic transmission. Such an approach has the potential to identify lead compounds for the development of novel drugs for the treatment of epilepsy, pathological pain states, Parkinson's disease, or other neurological disorders.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1056-8719
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
81-7
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pubmed:dateRevised |
2010-3-24
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pubmed:meshHeading |
pubmed-meshheading:14990332-Aniline Compounds,
pubmed-meshheading:14990332-Animals,
pubmed-meshheading:14990332-Baclofen,
pubmed-meshheading:14990332-Calcium,
pubmed-meshheading:14990332-Calcium Signaling,
pubmed-meshheading:14990332-Cells, Cultured,
pubmed-meshheading:14990332-Drug Evaluation, Preclinical,
pubmed-meshheading:14990332-Fetus,
pubmed-meshheading:14990332-GABA Antagonists,
pubmed-meshheading:14990332-Hippocampus,
pubmed-meshheading:14990332-Intracellular Fluid,
pubmed-meshheading:14990332-Isoquinolines,
pubmed-meshheading:14990332-Neurons,
pubmed-meshheading:14990332-Rats,
pubmed-meshheading:14990332-Rats, Sprague-Dawley,
pubmed-meshheading:14990332-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:14990332-Spectrometry, Fluorescence,
pubmed-meshheading:14990332-Xanthenes
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pubmed:articleTitle |
Measurement of intracellular Ca2+ in cultured rat embryonic hippocampal neurons using a fluorescence microplate reader: potential application to biomolecular screening.
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pubmed:affiliation |
School of Pharmacy, University of Queensland, St. Lucia Campus, Brisbane, Queensland, 4072, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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