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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-2-27
pubmed:abstractText
The vanilloid receptor subtype 1 (VR1)/(TRPV1), binding capsaicin, is a non-selective cation channel that recently has been shown in human keratinocytes in vitro and in vivo. However, a description of VR1 localization in other cutaneous compartments in particular cutaneous nerve fibers is still lacking. We therefore investigated VR1 immunoreactivity as well as mRNA and protein expression in a series (n = 26) of normal (n = 7), diseased (n = 13) [prurigo nodularis (PN) (n = 10), generalized pruritus (n = 1), and mastocytosis (n = 2)], and capsaicin-treated human skin (n = 6). VR1 immunoreactivity could be observed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands. Upon reverse transcriptase-polymerase chain reaction and Western blot analysis, VR1 was detected in mast cells and keratinocytes from human skin. In pruritic skin of PN, VR1 expression was highly increased in epidermal keratinocytes and nerve fibers, which was normalized after capsaicin application. During capsaicin therapy, a reduction of neuropeptides (substance P, calcitonin gene-related peptide) was observed. After cessation of capsaicin therapy, neuropeptides re-accumulated in skin nerves. In conclusion, VR1 is widely distributed in the skin, suggesting a major role for this receptor, e.g. in nociception and neurogenic inflammation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0906-6705
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-39
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed-meshheading:14987252-Adolescent, pubmed-meshheading:14987252-Adult, pubmed-meshheading:14987252-Aged, pubmed-meshheading:14987252-Animals, pubmed-meshheading:14987252-Biopsy, pubmed-meshheading:14987252-Brain, pubmed-meshheading:14987252-Calcitonin Gene-Related Peptide, pubmed-meshheading:14987252-Capillaries, pubmed-meshheading:14987252-Capsaicin, pubmed-meshheading:14987252-Cell Line, pubmed-meshheading:14987252-Child, pubmed-meshheading:14987252-Eccrine Glands, pubmed-meshheading:14987252-Epidermis, pubmed-meshheading:14987252-Epithelial Cells, pubmed-meshheading:14987252-Gene Expression, pubmed-meshheading:14987252-Hair Follicle, pubmed-meshheading:14987252-Humans, pubmed-meshheading:14987252-Mast Cells, pubmed-meshheading:14987252-Middle Aged, pubmed-meshheading:14987252-Nerve Fibers, pubmed-meshheading:14987252-Neurons, Afferent, pubmed-meshheading:14987252-Nociceptors, pubmed-meshheading:14987252-Pruritus, pubmed-meshheading:14987252-Rats, pubmed-meshheading:14987252-Receptors, Drug, pubmed-meshheading:14987252-Sebaceous Glands, pubmed-meshheading:14987252-Spinal Cord, pubmed-meshheading:14987252-Substance P
pubmed:year
2004
pubmed:articleTitle
Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures.
pubmed:affiliation
Department of Dermatology, University of Münster, Muenster, Germany. sonja.stander@uni-muenster.de
pubmed:publicationType
Journal Article