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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1992-9-17
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pubmed:abstractText |
The CACGTG G-box motif is a highly conserved DNA sequence that has been identified in the 5' upstream region of plant genes exhibiting regulation by a variety of environmental signals and physiological cues. Gel mobility shift assays using a panel of G-box oligonucleotides differing in their flanking sequences identified two types of binding activity (A and B) in a cauliflower nuclear extract. Competition gel retardation assays demonstrated that the two types of binding activity were distinct. Type A binding activity interacted with oligonucleotides designated as class I elements, whereas type B binding activity interacted strongly with class II elements and weakly with class I elements. A third class of elements, null elements, did not exhibit any detectable binding under our assay conditions. Gel retardation analysis of nonpalindromic hybrid G-box oligonucleotides indicated that hybrid elements of the same class exhibited binding affinity commensurate with the affinity of the weaker element, hybrid class I/II elements exhibited only type B binding, and hybrid class I/null and class II/null elements did not show any detectable binding activity. These binding activities can be explained by the affinity of bZip G-box binding homo- or heterodimer subunits for G-box half sites. These experiments led to a set of classification rules that can predict the binding activity of all reported plant G-box motifs containing the consensus hexameric core. Tissue- and/or development-specific expression of genes containing G-box motifs may be regulated by the affinity of G-box proteins for the different classes of G-box elements.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
485-96
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1498606-Base Sequence,
pubmed-meshheading:1498606-Brassica,
pubmed-meshheading:1498606-Cell Nucleus,
pubmed-meshheading:1498606-DNA,
pubmed-meshheading:1498606-DNA-Binding Proteins,
pubmed-meshheading:1498606-Molecular Sequence Data,
pubmed-meshheading:1498606-Protein Binding,
pubmed-meshheading:1498606-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1498606-Repetitive Sequences, Nucleic Acid
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pubmed:year |
1992
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pubmed:articleTitle |
Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding.
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pubmed:affiliation |
Laboratory for Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.
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pubmed:publicationType |
Journal Article
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