| pubmed-article:14984009 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14984009 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:14984009 | lifeskim:mentions | umls-concept:C0752312 | lld:lifeskim |
| pubmed-article:14984009 | lifeskim:mentions | umls-concept:C1150527 | lld:lifeskim |
| pubmed-article:14984009 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:14984009 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:14984009 | pubmed:dateCreated | 2004-2-26 | lld:pubmed |
| pubmed-article:14984009 | pubmed:abstractText | The aim of this study was to determine the pathway(s) by which ethanol activates mitogen-activated protein kinase (MAPK) signaling and to determine the role of Ca2+ in the signaling process. MAPK signaling was determined by assessing MAPK activity, measuring phosphorylated extracellular signaling-regulated kinase (pp 44 ERK-1 and pp 42 ERK-2) expression and ERK activity by measuring ERK-2-dependent phosphorylation of a synthetic peptide as a MAPK substrate in rat vascular smooth muscle cells. Ethanol activated extracellular signal-regulated kinase expression (ERK 1 and 2) could be observed when vascular smooth muscle cells (VSMCs) were stimulated for 5 min or less, but was inhibited when cells are treated for 10 min or more with 1-16 mM of ethanol. Maximum ethanol-induced MAPK activity was observed within 5 min with 4 or 8 mM. Ethanol stimulated MAPK activity was blocked by the protein kinase C (PKC) inhibitor (GF109203X) and epidermal growth factor (EGF) receptor antagonist (PD153035) by 41 +/- 24 and 34 +/- 12.3%, respectively. The calcium channel blocker, diltiazem and the chelating agent, BAPTA, reduced the activation of MAPK activity by ethanol, significantly. The data demonstrate that ethanol-stimulated MAPK expression is mediated partially through both the EGF-receptor and PKC intermediates and that activation through the PKC intermediate is calcium-dependent. | lld:pubmed |
| pubmed-article:14984009 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14984009 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14984009 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14984009 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14984009 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:14984009 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14984009 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14984009 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:14984009 | pubmed:issn | 0145-5680 | lld:pubmed |
| pubmed-article:14984009 | pubmed:author | pubmed-author:SmithHH | lld:pubmed |
| pubmed-article:14984009 | pubmed:author | pubmed-author:WilliamsSS | lld:pubmed |
| pubmed-article:14984009 | pubmed:author | pubmed-author:ShawBB | lld:pubmed |
| pubmed-article:14984009 | pubmed:author | pubmed-author:EidSS | lld:pubmed |
| pubmed-article:14984009 | pubmed:author | pubmed-author:WashingtonBB | lld:pubmed |
| pubmed-article:14984009 | pubmed:author | pubmed-author:GwathmeyJJ | lld:pubmed |
| pubmed-article:14984009 | pubmed:author | pubmed-author:MtshaliCC | lld:pubmed |
| pubmed-article:14984009 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14984009 | pubmed:volume | 49 | lld:pubmed |
| pubmed-article:14984009 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14984009 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14984009 | pubmed:pagination | 1351-6 | lld:pubmed |
| pubmed-article:14984009 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:14984009 | pubmed:meshHeading | pubmed-meshheading:14984009... | lld:pubmed |
| pubmed-article:14984009 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14984009 | pubmed:articleTitle | Ethanol-induced mitogen activated protein kinase activity mediated through protein kinase C. | lld:pubmed |
| pubmed-article:14984009 | pubmed:affiliation | Biomedical Research Center, Tennessee State University, Nashville, TN 37209, USA. bwashington@tnstate.edu | lld:pubmed |
| pubmed-article:14984009 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14984009 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:14984009 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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