Linked Life Data

14983999

Source:http://linkedlifedata.com/resource/pubmed/id/14983999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2004-2-26
pubmed:abstractText
Affymetrix microarray technology was used to characterize whole-hippocampus gene expression associated with in vivo N-methyl-D-aspartate (NMDA)-R-independent long-term potentiation (LTP) in the mossy fiber (MF)-Cornus Ammonis (CA)3 pathway of adult male F344 rats. Acute MF responses were evoked by stimulation of the MF bundle and recorded in stratum lucidum of CA3. Following recording of baseline responses at 0.05 Hz, animals received either CPP (NMDA-R antagonist, 10 mg/kg) or naloxone (opioid-R antagonist, 10 mg/kg). LTP was induced by two 100 Hz 1-sec trains at the intensity sufficient to evoke 50% of the maximal response. Responses were collected for an additional hour. In controls, MF responses were collected at 0.05 Hz for 1 hr, but 100 Hz trains were not delivered. Hippocampi were harvested prior to total RNA isolation. Fragmented cRNA was hybridized to a rat U34 neurobiology array. F344 rats exhibited characteristic LTP in the presence of CPP and LTP blockade in the presence of naloxone. As a result, genes associated with both NMDA-independent LTP and naloxone-induced blockade were identified. These include genes involved in transmitter transport, intracellular messengers, growth factors and ion channels. Up-regulated include NMDA-R2D, neuropeptide Y (NPY), proenkephalin, BDNF and NGFR. Down-regulated genes include IGF-1 and GABA-B.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0145-5680
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1281-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Gene expression associated with in vivo induction of early phase-long-term potentiation (LTP) in the hippocampal mossy fiber-Cornus Ammonis (CA)3 pathway.
pubmed:affiliation
University of Texas at San Antonio, Department of Biology, 6900 N. Loop 1604 West, San Antonio, TX 78249, USA. kthompson@utsa.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't