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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0031437,
umls-concept:C0032214,
umls-concept:C0035820,
umls-concept:C0040132,
umls-concept:C0069676,
umls-concept:C0185117,
umls-concept:C0205281,
umls-concept:C0543431,
umls-concept:C0596138,
umls-concept:C0600138,
umls-concept:C0694890,
umls-concept:C1948023,
umls-concept:C2911684
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pubmed:issue |
12
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pubmed:dateCreated |
2004-3-18
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pubmed:abstractText |
Papillary thyroid carcinomas are characterized by rearrangements of the RET receptor tyrosine kinase generating RET/PTC oncogenes. Here we show that osteopontin (OPN), a secreted glycoprotein, is a major RET/PTC-induced transcriptional target in PC Cl 3 thyroid follicular cells. OPN upregulation depended on the integrity of the RET/PTC kinase and tyrosines Y1015 and Y1062, two major RET/PTC autophosphorylation sites. RET/PTC also induced a strong overexpression of CD44, a cell surface signalling receptor for OPN. Upregulation of CD44 was dependent on RET/PTC Y1062, as well. Constitutive OPN overexpression or treatment with exogenous recombinant OPN sharply increased proliferation, Matrigel invasion and spreading in collagen gels of RET/PTC-transformed PC Cl 3 cells. These effects were impaired by the treatment of PC Cl 3-RET/PTC cells with OPN- and CD44-locking antibodies. Thus, RET/PTC signalling triggers an autocrine loop involving OPN and CD44 that sustains proliferation and invasion of transfomed PC Cl 3 thyrocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type I,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Combinations,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/Osteopontin,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SPP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/matrigel
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0950-9232
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pubmed:author |
pubmed-author:BasoloFulvioF,
pubmed-author:CastelloneMaria DomenicaMD,
pubmed-author:CelettiAngelaA,
pubmed-author:CiraficiAnna MariaAM,
pubmed-author:CurcioFrancescoF,
pubmed-author:FuscoAlfredoA,
pubmed-author:GianniniRiccardoR,
pubmed-author:GuarinoValentinaV,
pubmed-author:KruhofferMogensM,
pubmed-author:MedicoEnzoE,
pubmed-author:MelilloRosa MarinaRM,
pubmed-author:OrntoftTorben FTF,
pubmed-author:SantoroMassimoM
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2188-96
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14981541-Antigens, CD44,
pubmed-meshheading:14981541-Carcinoma, Papillary,
pubmed-meshheading:14981541-Cell Division,
pubmed-meshheading:14981541-Cell Line, Tumor,
pubmed-meshheading:14981541-Cell Transformation, Neoplastic,
pubmed-meshheading:14981541-Collagen,
pubmed-meshheading:14981541-Collagen Type I,
pubmed-meshheading:14981541-DNA,
pubmed-meshheading:14981541-Drug Combinations,
pubmed-meshheading:14981541-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:14981541-Humans,
pubmed-meshheading:14981541-Laminin,
pubmed-meshheading:14981541-Neoplasm Invasiveness,
pubmed-meshheading:14981541-Osteopontin,
pubmed-meshheading:14981541-Phenotype,
pubmed-meshheading:14981541-Phosphorylation,
pubmed-meshheading:14981541-Protein Structure, Tertiary,
pubmed-meshheading:14981541-Proteoglycans,
pubmed-meshheading:14981541-Proto-Oncogene Proteins,
pubmed-meshheading:14981541-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:14981541-Recombinant Proteins,
pubmed-meshheading:14981541-Sialoglycoproteins,
pubmed-meshheading:14981541-Signal Transduction,
pubmed-meshheading:14981541-Thyroid Neoplasms,
pubmed-meshheading:14981541-Tyrosine
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pubmed:year |
2004
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pubmed:articleTitle |
Autocrine stimulation by osteopontin plays a pivotal role in the expression of the mitogenic and invasive phenotype of RET/PTC-transformed thyroid cells.
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pubmed:affiliation |
Dipartimento di Biologia e Patologia Cellulare e Molecolare, University Federico II c/o Istituto di Endocrinologia ed Oncologia Sperimentale del CNR, Naples, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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