Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-2-23
pubmed:abstractText
Amplification within chromosome arm 11q involving the mixed-lineage leukemia gene (MLL) locus is a rare but recurrent aberration in acute myeloid leukemia and myelodysplastic syndrome (AML/MDS). We and others have observed that 11q amplifications in most AML/MDS cases have not been restricted to the chromosomal region surrounding the MLL gene. Therefore, we implemented a strategy to characterize comprehensively 11q amplicons in a series of 13 AML/MDS patients with MLL amplification. Analysis of 4 of the 13 cases by restriction landmark genomic scanning in combination with virtual genome scan and by matrix-based comparative genomic hybridization demonstrated that the 11q amplicon in these four cases consisted of at least three discontinuous sequences derived from different regions of the long arm of chromosome 11. We defined a maximally 700-kb sequence around the MLL gene that was amplified in all cases. Apart from the core MLL amplicon, we detected two additional 11q regions that were coamplified. Using fluorescence in situ hybridization (FISH) analysis, we demonstrated that sequences in 11q13.5 and 11q23-24 were amplified in 8 of 13 and 10 of 12 AML/MDS cases, respectively. Both regions harbor a number of potentially oncogenic genes. In all 13 cases, either one or both of these regions were coamplified with the MLL amplicon. Thus, we demonstrated that 11q amplicons in AML/MDS patients display a complex organization and have provided evidence for coamplification of two additional regions on the long arm of chromosome 11 that may harbor candidate target genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1045-2257
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14978788-Acute Disease, pubmed-meshheading:14978788-Aged, pubmed-meshheading:14978788-Aged, 80 and over, pubmed-meshheading:14978788-Chromosomes, Human, Pair 11, pubmed-meshheading:14978788-Cytogenetic Analysis, pubmed-meshheading:14978788-DNA, Neoplasm, pubmed-meshheading:14978788-DNA Probes, pubmed-meshheading:14978788-DNA-Binding Proteins, pubmed-meshheading:14978788-Female, pubmed-meshheading:14978788-Gene Amplification, pubmed-meshheading:14978788-Gene Dosage, pubmed-meshheading:14978788-Genetic Markers, pubmed-meshheading:14978788-Humans, pubmed-meshheading:14978788-In Situ Hybridization, Fluorescence, pubmed-meshheading:14978788-Leukemia, Myeloid, pubmed-meshheading:14978788-Male, pubmed-meshheading:14978788-Middle Aged, pubmed-meshheading:14978788-Myelodysplastic Syndromes, pubmed-meshheading:14978788-Myeloid-Lymphoid Leukemia Protein, pubmed-meshheading:14978788-Nucleic Acid Hybridization, pubmed-meshheading:14978788-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:14978788-Polymerase Chain Reaction, pubmed-meshheading:14978788-Proto-Oncogenes, pubmed-meshheading:14978788-Restriction Mapping, pubmed-meshheading:14978788-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
Distinct sequences on 11q13.5 and 11q23-24 are frequently coamplified with MLL in complexly organized 11q amplicons in AML/MDS patients.
pubmed:affiliation
Institut für Medizinische Biologie, Universität Wien, Wien, Austria.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't