Source:http://linkedlifedata.com/resource/pubmed/id/14978093
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2004-2-23
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| pubmed:abstractText |
IL-12 has been reported to affect thymic T cell selection, but the role of IL-12 in thymic involution has not been studied. We found that in vivo, IL-12b knockout (IL-12b(-/-)) mice exhibited accelerated thymic involution compared with wild-type (WT) B6 mice. This is characterized by an increase in thymocytes with the early development stage phenotype of CD25(-)CD44(+)CD4(-)CD8(-) in aged IL-12b(-/-) mice. Histologically, there were accelerated degeneration of thymic extracellular matrix and blood vessels, a significantly decreased thymic cortex/medulla ratio, and increased apoptotic cells in aged IL-12b(-/-) mice compared with WT mice. There was, however, no apparent defect in thymic structure and thymocyte development in young IL-12(-/-) mice. These results suggest the importance of IL-12 in maintaining thymic integrity and function during the aging process. Surprisingly, in WT B6 mice, there was no age-related decrease in the levels of IL-12 produced from thymic dendritic cells. Stimulation of thymocytes with IL-12 alone also did not enhance the thymocyte proliferative response in vitro. IL-12, however, provided a strong synergistic effect to augment the IL-7 or IL-2 induced thymocyte proliferative response, especially in aged WT and IL-12b(-/-) mice. Our data strongly support the role of IL-12 as an enhancement cytokine, which acts through its interactions with other cytokines to maintain thymic T cell function and development during aging.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12 Subunit p40,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
172
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2909-16
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:14978093-Adjuvants, Immunologic,
pubmed-meshheading:14978093-Aging,
pubmed-meshheading:14978093-Animals,
pubmed-meshheading:14978093-Apoptosis,
pubmed-meshheading:14978093-Cell Division,
pubmed-meshheading:14978093-Cells, Cultured,
pubmed-meshheading:14978093-Drug Synergism,
pubmed-meshheading:14978093-Female,
pubmed-meshheading:14978093-Interleukin-12,
pubmed-meshheading:14978093-Interleukin-12 Subunit p40,
pubmed-meshheading:14978093-Interleukin-2,
pubmed-meshheading:14978093-Interleukin-7,
pubmed-meshheading:14978093-Mice,
pubmed-meshheading:14978093-Mice, Inbred C57BL,
pubmed-meshheading:14978093-Mice, Knockout,
pubmed-meshheading:14978093-Protein Subunits,
pubmed-meshheading:14978093-Signal Transduction,
pubmed-meshheading:14978093-T-Lymphocyte Subsets,
pubmed-meshheading:14978093-Thymus Gland
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| pubmed:year |
2004
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| pubmed:articleTitle |
IL-12 inhibits thymic involution by enhancing IL-7- and IL-2-induced thymocyte proliferation.
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| pubmed:affiliation |
Department of Pathology, Veterans Administration Medical Center, Birmingham, AL 35233, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|