Linked Life Data

14976526

Source:http://linkedlifedata.com/resource/pubmed/id/14976526

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-3-19
pubmed:abstractText
Surrogate light chains consisting of VpreB (CD179a) and lambda5 (CD179b) are expressed in precursor B cells lacking a complete form of immunoglobulin and are thought to act as substitutes for conventional light chains. Upon differentiation to immature and mature B cells, CD179a/b disappear and are replaced with conventional light chains. Thus, these molecules may be useful as essential markers of precursor B cells. To examine the expression of the surrogate light-chain components CD179a and CD179b in precursor B-cell lymphoblastic lymphoma, we analyzed tissue sections using immunohistochemistry techniques. Among a number of monoclonal antibodies for the surrogate light chains, VpreB8 and SL11 were found to detect CD179a and CD179b, respectively, in acetone-fixed fresh frozen sections. Moreover, we also observed VpreB8 staining in formalin-fixed, paraffin-embedded sections. Using these antibodies, we found that CD179a/b were specifically expressed in precursor B-cell lymphoblastic lymphomas, but not in mature B-cell lymphomas in childhood. Furthermore, other pediatric tumors that must be included in a differential diagnosis of precursor B-cell lymphoblastic lymphoma, including precursor T-cell lymphoblastic lymphoma, extramedullary myeloid tumors, and Ewing sarcoma, were also negative for both CD179a and CD179b. Our data indicate that CD179a and CD179b may be important markers for the immunophenotypic diagnosis of precursor B-cell lymphoblastic lymphomas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0893-3952
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
423-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:14976526-Adolescent, pubmed-meshheading:14976526-B-Lymphocytes, pubmed-meshheading:14976526-Cell Line, Tumor, pubmed-meshheading:14976526-Child, pubmed-meshheading:14976526-Child, Preschool, pubmed-meshheading:14976526-Flow Cytometry, pubmed-meshheading:14976526-Humans, pubmed-meshheading:14976526-Immunoenzyme Techniques, pubmed-meshheading:14976526-Immunoglobulin Light Chains, pubmed-meshheading:14976526-Immunoglobulin Light Chains, Surrogate, pubmed-meshheading:14976526-Infant, pubmed-meshheading:14976526-Lymphoma, B-Cell, pubmed-meshheading:14976526-Male, pubmed-meshheading:14976526-Membrane Glycoproteins, pubmed-meshheading:14976526-Precursor Cell Lymphoblastic Leukemia-Lymphoma, pubmed-meshheading:14976526-Sensitivity and Specificity, pubmed-meshheading:14976526-Tumor Markers, Biological
pubmed:year
2004
pubmed:articleTitle
Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma.
pubmed:affiliation
Department of Developmental Biology, National Research Institute for Child Health and Development, Japan. nkiyokawa@nch.go.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't