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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-9-10
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pubmed:abstractText |
We have studied the binding of Al3+ to human serum apotransferrin (80 kDa) and recombinant N-lobe human apotransferrin (40 kDa) in 0.1 M-sodium bicarbonate solution at a pH meter reading in 2H2O (pH*) of 8.8 using 1H n.m.r. spectroscopy. The results show that for the intact protein, preferential binding of Al3+ to the N-lobe occurs. Molecular modelling combined with an analysis of ring-current-induced shifts suggest that n.m.r. spectroscopy can be used to probe hinge bending processes which accompany metal uptake in solution.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-100104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-1674316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-2195850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-2330032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-2334724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-2536903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-2843602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-3179277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-3663138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-444649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-562778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497609-999884
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
285 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
711-4
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1497609-Aluminum,
pubmed-meshheading:1497609-Amino Acid Sequence,
pubmed-meshheading:1497609-Apoproteins,
pubmed-meshheading:1497609-Binding Sites,
pubmed-meshheading:1497609-Humans,
pubmed-meshheading:1497609-Hydrogen-Ion Concentration,
pubmed-meshheading:1497609-Magnetic Resonance Spectroscopy,
pubmed-meshheading:1497609-Models, Molecular,
pubmed-meshheading:1497609-Molecular Sequence Data,
pubmed-meshheading:1497609-Molecular Structure,
pubmed-meshheading:1497609-Recombinant Proteins,
pubmed-meshheading:1497609-Transferrin
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pubmed:year |
1992
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pubmed:articleTitle |
Uptake of Al3+ into the N-lobe of human serum transferrin.
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pubmed:affiliation |
Department of Chemistry, Birkbeck College, University of London, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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