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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1992-9-10
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pubmed:abstractText |
Incubation of digitonin-permeabilized bovine chromaffin cells in the absence of Ca2+ results in a loss of both cytosolic proteins and Ca(2+)-dependent secretion. Addition of these leaked proteins prevents this loss of secretory activity. We have purified a protein from an extract of bovine adrenal medulla which can partially prevent this loss of Ca(2+)-dependent secretion. Antibody against this protein inhibited the ability of leaked chromaffin-cell proteins to prevent the loss of Ca(2+)-dependent secretion. Sequence analysis showed it to have sequence identity with bovine brain 14-3-3 protein. These results demonstrate that 14-3-3 protein makes a significant contribution to the ability of leaked chromaffin-cell proteins to maintain secretory activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-1538762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-1646643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-1671102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-1827411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-1832677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-2002353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-2143472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-2174438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-2295612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-2526299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-2674132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-2885229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-3156373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-3156762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-3169247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-3184176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-3500275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-3680269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-403997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-533865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-6113235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-6300108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-6833287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-6884518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-7038050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1497607-7062063
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
285 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
697-700
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1497607-14-3-3 Proteins,
pubmed-meshheading:1497607-Adrenal Medulla,
pubmed-meshheading:1497607-Amino Acid Sequence,
pubmed-meshheading:1497607-Animals,
pubmed-meshheading:1497607-Antibodies,
pubmed-meshheading:1497607-Brain Chemistry,
pubmed-meshheading:1497607-Calcium,
pubmed-meshheading:1497607-Cattle,
pubmed-meshheading:1497607-Cells, Cultured,
pubmed-meshheading:1497607-Chromaffin System,
pubmed-meshheading:1497607-Cytosol,
pubmed-meshheading:1497607-Molecular Sequence Data,
pubmed-meshheading:1497607-Nerve Tissue Proteins,
pubmed-meshheading:1497607-Norepinephrine,
pubmed-meshheading:1497607-Tyrosine 3-Monooxygenase
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pubmed:year |
1992
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pubmed:articleTitle |
Anti-(14-3-3 protein) antibody inhibits stimulation of noradrenaline (norepinephrine) secretion by chromaffin-cell cytosolic proteins.
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pubmed:affiliation |
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
Comparative Study
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